Wiley-VCH - Electrophoresis in Practice

Westermeier, Reiner

Electrophoresis in Practice

3. Edition - January 2001
69.- Euro / 134.95 DM / 120.- SFR
2001. XVIII, 350 Pages, Hardcover
ISBN 3-527-30300-6 - Wiley-VCH, Weinheim

Short description

This is your "Laboratory Guide" for successful electrophoretic separations! Organized in two parts it gives the reader a thorough presentation of the fundamentals followed by a detailed description of 15 of the most common methods currently in use. The Third Edition now includes the latest developments in 2D-electrophoresis as well as an overview of the proteome analysis methodology.

From the contents

PART I: FUNDAMENTALS
Introduction
Electrophoresis
Isotachophoresis
Isoelectric Focusing
Blotting
Interpretation of Electropherograms
Proteome Analysis
Instrumentation
Equipment for Part II
PART II: METHODS
Method 1: PAGE of Dyes
Method 2: Agarose and Immuno Electrophoresis
Method 3: Titration Curve Analysis
Method 4: Native PAGE in Amphoteric Buffers
Method 5: Agarose IEF
Method 6: PAGIEF in Rehydrated Gels
Method 7: Horizontal SDS-PAGE
Method 8: Vertical PAGE
Method 9: Semi-dry Blotting of Proteins
Method 10: IEF in Immobilized pH Gradients
Method 11: High-resolution 2D Electrophoresis
Method 12: PAGE of Double Stranded DNA
Method 13: Native PAGE of Single Stranded DNA
Method 14: Denaturing Gradient Gel Electrophoresis
Method 15: Denaturing PAGE of DNA
Appendix A: Trouble-shooting Guide
Appendix B: References
Index

Principle of Electrophoresis - (Animation)
Principle of Isotachophoresis - (Animation)
Principle of Isoelectric Focusing - (Animation)
Principle of Electroendosmosis - (Animation)
Principle of Disc Electrophoresis - (Animation)
Principle of 2-D Electrophoresis - (Animation)
Principle of Carrier Ampholyte Generated pH Gradients - (Animation)

Animations of Electrophoretic Separations (Basic Principles)
Please note: The graphics of the animations are adjusted to a screen resolution of 1280 x 1024 pixels. This resolution is needed in order to display the full size of the graphics on your computer screen.

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