Wiley-VCH - Proteomics in Practice

Westermeier, Reiner / Naven, Tom

Proteomics in Practice

A Laboratory Manual of Proteome Analysis

1. Edition - May 2002
2002. XXIV, 318 Pages, Hardcover
ISBN 3-527-30354-5 - Wiley-VCH, Weinheim

Short description

A combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries. Intended for all those wishing to go beyond the theoretical aspects of proteome analysis, this book is targeted at research groups within academia and industry, course instructors, research assistants and graduate students.

From the contents

Foreword
Preface
Abbreviations, Symbols, Units
Glossary of Terms

PART I: PROTEOMICS TECHNOLOGY
Introduction
Expression Proteomics
Two-dimensional Electrophoresis
Spot Handling
Mass Spectrometry
Protein Identification by Database Searching
Methods of Proteomics

PART II: COURSE MANUAL
Step 1: Sample Preparation
Step 2: Isoelectric Focusing
Step 3: SDS Polyacrylamide Gel Electrophoresis
Step 4: Staining of the Gels
Step 5: Scanning of Gels and Image Analysis
Step 6: 2D DIGE
Step 7: Spot Excision
Step 8: Sample Destaining
Step 9: In-gel Digestion
Step 10: Microscale Purification
Step 11: Chemical Derivatisation of the Peptide Digest
Step 12: MS Analysis
Step 13: Calibration of the MALDI-ToF MS
Step 14: Preparing for a Database Search
Step 15: PMF Database Search Unsuccessful

Appendices:
Trouble Shooting Guide
References

Principle of 2-D Electrophoresis - (Animation)
MALDI - ToF Mass Spectrometer - (Animation)
Reflectron - (Animation)
Post Source Decay Analysis with Reflectron - (Animation)
Electrospray Ionization - (Animation)

Animations of Proteomic Separations (Basic Principles)
Please note: The graphics of the animations are adjusted to a screen resolution of 1280 x 1024 pixels. This resolution is needed in order to display the full size of the graphics on your computer screen.

Erratum (PDF)

Addendum (PDF)