Directed Evolution of Selective Enzymes
Catalysts for Organic Chemistry and Biotechnology
1. Edition November 2016
X, 308 Pages, Hardcover
16 tables
Handbook/Reference Book
Short Description
Written by one of the most authoritative organic chemists worldwide, this book combines techniques from nature with synthetic organic approaches to bring the best results possible. An overview of current research with a look at future industrial applications.
Further versions
Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods.
Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments.
Selection versus screening in directed evolution
Gene mutagenesis methods
Strategies for applying gene mutagenesis methods
Selected examples of directed evolution of enzymes with emphasis on stereo- and regioselectivity, substrate scope and/or activity
Directed evolution of enzyme robustness
Directed evolution of promiscuity: Artificial enzymes as catalysts in organic chemistry
Learning from directed evolution
