 Double your protein IDs to make sure it's the one. (hmm, scans a bit rough). Platelets make an interesting proteome target because when mature, they are anucleate. Traditionally known for participating in hemostasis and clotting, new information points to many other platelet functions, including immune response, bone metastasis, and liver regeneration. Yu et al. wanted to explore this further in an appropriate animal model system. Rat was the preferred animal, with a strong resemblance to humans in cardiovascular problems. A problem at the time the work was done was no rat genome sequence. To carry out the research, the platelet proteomes were compared extensively: multi-step protein purification, various modes of LC-MS/MS, two different algorithms for MS/MS spectrum interpretation. Some 837 unique proteins were identified by at least two unique peptide matches each. /tl
To appear in PROTEOMICS Issue 13.
Global analysis of the rat and human platelet proteome - the molecular blueprint for illustrating multi-functional platelets and cross-species function evolution
Yu, Y. et al., Proteomics 2010, 10, DOI: 10.1002/pmic. 200900271
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