|  | Zhang, Wei / Shmulevich, Ilya / Astola, Jaakko
Microarray Quality Control
 1. Auflage März 2004
112,- Euro
2004. 136 Seiten, Hardcover
- Handbuch/Nachschlagewerk -
ISBN 978-0-471-45344-4 - John Wiley & Sons
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| Kurzbeschreibung
This book covers the breadth of issues encompassed by quality control in microarray studies, including quality of biological samples, quality of DNA, hybridization protocols, scanning, data acquisition, image analysis, and data analysis. Its unique focus on quality control addresses a serious knowledge gap amongst the interdisciplinary group of workers--biologists, mathematicians, statisticians, engineers, physicians, and computational scientists--involved in microarray studies. This book provides the only systematic treatment of quality control beginning with experimental design through to interpreting results.
Aus dem Inhalt
Foreword.
Preface.
1. Quality of Biological Samples.
1.1 Tissue Acquisition, Handling, and Storage.
1.2 Pathological Evaluation.
1.3 Tissue heterogeneity and Laser Capture Microdissection.
1.4 Summary.
2. Microarray Production: Quality of DNA and Printing.
2.1 Quality Control for cDNA Probes.
2.2 Long-oligo Arrays.
2.3 Slide Coating.
2.4 Slide Autofluorescence.
2.5 Printing Quality Control.
2.6 Summary.
3. Quality of Microarray Hybridization.
3.1 RNA and cDNA Labeling Quality.
3.2 Specificity versus Sensitivity.
3.3 Amplification Strategies.
3.4 Indirect Labeling.
3.5 Automation of Microarray Hybridization.
3.6 Hybridization Reference.
3.7 Validation of Microarray Experiments.
3.8 Summary.
4. Scanners and Data Acquisition.
4.1 Basic Principles of Scanners.
4.2 Basic Principles of Imagers.
4.3 Calibration and PMT Gain.
4.4 Characteristics of Different Noise Sources.
5. Image Analysis.
5.1 Grid Alignment.
5.2 Spot Segmentation.
5.3 Extracting Information.
5.4 Appendix: Image Processing.
6. Quality Control in Data Analysis.
6.1 Normalization.
6.2 Replication.
6.3 Missing Values.
6.4 Data Management Issues.
6.5 Small-Sample-Size Issues.
Index.
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