Kurzbeschreibung This book uniquely provides basic knowledge and principles of proteomic bioinformatics and mass spectrometry. Computational Methods for Mass Spectrometry Proteomics covers the bioinformatics problems and how they are solved, which types of programs are used and the different principles / algorithms underlying these programs. A description of the instruments used is also given. The book includes many examples to help make the subject practical and accessible.
Aus dem Inhalt Contents
Preface
1 Protein, Proteome, and Proteomics
2 Protein Separation - 2D Gel Electrophoresis
dimension
3 Protein Digestion
4 Peptide Separation - HPLC
phase chromatography
5 Fundamentals of Mass Spectrometry
6 Mass Spectrometry - MALDI-TOF
7 Protein Identification and Characterization by MS
8 Tandem MS or MS/MS Analysis
9 Fragmentation Models
10 Identification and Characterization by MS/MS
11 Spectral Comparisons
12 Sequencial Comparison - de novo Sequencing
13 Database Searching for De Novo Sequences
flanking amino acids
14 Large-Scale Proteomics
15 Quantitative Mass Spectrometry-Based Proteomics
