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Komatsuzaki, Tamiki / Kawakami, M. / Takahashi, Satoshi / Yang, Haw / Silbey, Robert J. / Rice, Stuart A.
Advances in Chemical Physics
Volume 146: Single Molecule Biophysics. Experiments and Theory
Advances in Chemical Physics
(Volume 146)
Edited by Dinner, Aaron R.

1. Edition January 2012
195.- Euro
2012. 512 Pages, Hardcover
- Wiley & Sons Ltd -
ISBN 978-1-118-05780-3 - John Wiley & Sons

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Short description
Single-molecule experiments have provided fresh perspectives to questions such as how proteins fold to specific conformations from highly heterogeneous structures, how signal transductions take place on the molecular level, and how proteins behave in membranes and live cells. This book focuses mainly on three experimental approaches: single-molecule fluorescence based mainly on fluorescence resonance energy transfer (FRET), atomic force microscopy (AFM) and diffracted X-ray tracking (DXT), and their applications to important biological phenomena. In addition, discussion is provided on the theoretical progress in the single-molecule data analyses. For students and professionals in chemical physics and physical chemistry from academia, government, and industries.

From the contents
Preface xiii

Part One Developments on Single-Molecule Experiments

Staring at a Protein: Ensemble and Single-Molecule Investigations on Protein-Folding Dynamics 3
By Satoshi Takahashi and Kiyoto Kamagata

Single-Molecule FRET of Protein-Folding Dynamics 23
By Daniel Nettels and Benjamin Schuler

Quantitative Analysis of Single-Molecule FRET Signals and its Application to Telomere DNA 49
By Kenji Okamoto and Masahide Terazima

Force to Unbind Ligand-Receptor Complexes and the Internal Rigidity of Globular Proteins Probed by Single-Molecule Force Spectroscopy 71
By Atsushi Ikai, Rehana Afrin, and Hiroshi Sekiguchi

Recent Advances in Single-Molecule Biophysics with the Use of Atomic Force Microscopy 89
By Masaru Kawakami and Yukinori Taniguchi

Dynamical Single-Molecule Observations of Membrane Protein Using High-Energy Probes 133
By Yuji C. Sasaki

Single-Molecular Gating Dynamics for the KcsA Potassium Channel 147
By Shigetoshi Oiki, Hirofumi Shimizu, Masayuki Iwamoto, and Takashi Konno

Static and Dynamic Disorder in IN VITRO Reconstituted Receptor-Adaptor Interaction 195
By Hiroaki Takagi, Miki Morimatsu, and Yasushi Sako

Part Two Developments on Single-Molecule Theories and Analyses

Change-Point Localization and Wavelet Spectral Analysis of Single-Molecule Time Series 219
By Haw Yang

Theory of Single-Molecule FRET Efficiency Histograms 245
By Irina V. Gopich and Attila Szabo

Multidimensional Energy Landscapes in Single-Molecule Biophysics 299
By Akinori Baba and Tamiki Komatsuzaki

Generalized Michaelis-Menten Equation for Conformation Modulated Monomeric Enzymes 329
By Jianlan Wu and Jianshu Cao

Making it Possible: Constructing a Reliable Mechanism from a Finite Trajectory 367
By Ophir Flomenbom

Free Energy Landscapes of Proteins: Insights from Mechanical Probes 395
By Zu Thur Yew, Peter D. Olmsted, and Emanuele Paci

Mechanochemical Coupling Revealed by the Fluctuation Analysis of Different Biomolecular Motors 419
By Hiroaki Takagi and Masatoshi Nishikawa

Author Index 437

Subject Index 467



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