Mass Spectrometry in Sports Drug Testing
Characterization of Prohibited Substances and Doping Control Analytical Assays
Wiley-Interscience Series on Mass Spectrometry
1. Edition July 2010
376 Pages, Hardcover
Wiley & Sons Ltd
Further versions
Enables you to detect, identify, and characterize hundreds of
drugs that may be used by athletes
Mass spectrometry has become essential to sports drug testing.
This book examines both the principles of sports drug testing and
the use of mass spectrometry techniques and mass spectral data to
detect, identify, and characterize hundreds of known and unknown
drugs that athletes may use to enhance their performance. The
author provides a detailed overview of the mass spectrometry of
numerous classes of therapeutics and agents, various analyzers to
detect low- and high-molecular weight drugs, as well as techniques
to discriminate between endogenously produced and synthetically
derived compounds.
Mass Spectrometry in Sports Drug Testing begins with a
full chapter dedicated to the history of sports drug testing. Next,
the book provides the principles and techniques needed to maximize
the specificity and sensitivity of mass spectrometric assays,
including:
* Detailed, step-by-step assays with sample preparation
* Discussion of both chromatographic separation and mass
spectrometric analysis
* Characterization of analytes in order to unequivocally
identify banned substances
* Mass spectrometric behavior of low- and high-molecular weight
analytes
Throughout the book, descriptive examples illustrate the
principles, advantages, and limitations of different assays.
Mass Spectrometry in Sports Drug Testing not only sets
forth the role mass spectrometry plays in detecting drug use among
athletes, it also adds new insights into the health and ethical
issues of doping in sports.
Acknowledgments.
1 History of Sports Drug Testing.
1.1 Historical Attempts of Artificial Performance
Enhancement.
1.2 Background and Rationale of Doping Controls.
1.3 Early Detection Methods: Possibilities and Limitations of
Assays Without Mass Spectrometry.
1.4 Introduction of Mass Spectrometry to Doping Control
Analysis.
References.
2. Mass Spectrometry and the List of Prohibited Substances
and Methods of Doping.
2.1 Criteria for the Mass Spectrometric Identification of
Prohibited Compounds.
2.2 Modern Mass Spectrometers in Doping Controls: Advantages and
Disadvantages of Available Techniques.
References.
3. Structure Characterization of Low Molecular Weight Target
Analytes --Electron Ionization.
3.1 Stimulants.
3.2 Narcotics.
3.3 Anabolic Androgenic Steroids.
3.4 Selective Androgen Receptor Modulators (SARMs).
3.5 Diuretics.
3.6 beta2-Agonists.
3.7 beta-Receptor Blocking Agents.
3.8 Calcium-Channel Modulators (RYCALS).
3.9 Carbohydrate-Based Agents.
References.
4. Structure Characterization of Low Molecular Weight Target
Analytes: Electrospray Ionization.
4.1 Stimulants.
4.2 Narcotics.
4.3 Anabolic Androgenic Steroids.
4.4 Selective Androgen Receptor Modulators (SARMs).
4.5 Diuretics.
4.6 beta2-Agonists.
4.7 Calcium-Channel Modulators (RYCALS).
4.8 Peroxisome-Proliferator Activated Receptor-delta
(PPARdelta) And Adenosine Monophosphate Activated Protein Kinase
(AMPK) Agonists.
4.9 Hypoxia-Inducible Factor (HIF)-Stabilizers And Sirtuin
Activators.
4.10 beta-Receptor Blocking Agents.
4.11 Glucuronic Acid and Sulfate Conjugates of Target
Analytes.
References.
5. Structure Characterization of High Molecular Weight Target
Analytes: Electrospray Ionization.
5.1 Human Chorionic Gonadotrophin (hCG).
5.2 Erythropoietins (EPO).
5.3 Synacthen.
5.4 Insulins.
5.5 Hemoglobin-Based Oxygen Carriers (HBOCs).
5.6 Human Growth Hormone (hGH).
5.7 Sermorelin (Geref).
5.8 Insulin-Like Growth Factor-1 (IGF-1).
5.9 Gonadorelin (LHRH).
References.
6. Modern Mass Spectrometry-Based Analytical Assays.
6.1 GC-MS and Isotope Ratio Mass Spectrometry.
6.2 LC-MS/MS.
References.
7. Limitations and Perspectives of Mass Spectrometry-Based
Procedures in Doping Control Analysis.
7.1 Recombinant Biomolecules.
7.2 Unknown Compounds.
7.3 Profiling of Urine and/or Blood.
7.4 Alternative Specimens.
References.
Index.
textbook for anyone involved in the analytical characterization of
illicit substances and abused drugs." (Anal Bioanal
Chem, 2011)
