1. Edition - May 2010
142.- Euro
2010. 478 Pages, Hardcover
ISBN-10: 0-470-51974-6
ISBN-13: 978-0-470-51974-5 - John Wiley & Sons






Sample Chapter

Short description
With extensive coverage of synthesis techniques and applications, this text describes chemical biology techniques which have gained significant impetus during the last five years. It focuses on the methods for obtaining modified and native nucleic acids, and their biological applications. The topics covered include: synthesis and site-directed modifications of DNA and RNA; DNA-templated synthesis; antisense nucleic acids; RNA interferences; photoregulated nucleic acids; ribose modifications; functionalized DNA; aptamers; therapeutic and diagnostic applications; and chemical biology applied to the RNA world hypothesis.

From the contents
Foreword.

Preface.

List of Contributors.

1 Chemical Synthesis of Modified RNA (Claudia Hobartner and Falk Wachowius).

2 Expansion of the Genetic Alphabet in Nucleic Acids by Creating New Base Pairs (Ichiro Hirao and Michiko Kimoto).

3 Chemical Biology of DNA Replication: Probing DNA Polymerase Selectivity Mechanisms with Modified Nucleotides (Andreas Marx).

4 Nucleic Acid-templated Chemistry (Michael Oberhuber).

5 Chemical Biology of Peptide Nucleic Acids (PNAs) (Peter E. Nielsen).

6 The Interactions of Small Molecules with DNA and RNA (Yun Xie, Victor K. Tam and Yitzhak Tor).

7 The Architectural Motifs of Folded RNAs (Valerie Fritsch and Eric Westhof).

8 Genesis and Biological Applications of Locked Nucleic Acids (LNAs) (Harleen Kaur and Souvik Maiti).

9 Small Non-coding RNA in Bacteria (Sabine Brantl).

10 MicroRNA-guided Gene Silencing (Gunter Meister).

11 Nucleic Acid-based Therapies (Britta Hoehn and John J. Rossi).

12 Innate Immune Recognition of Nucleic Acids (Stefan Bauer).

13 Light-responsive Nucleic Acids for the Spatiotemporal Control of Biological Processes (Alexander Heckel and Gunter Mayer).

14 DNA Methylation (Albert Jeltsch and Renata Z. Jurkowska).

15 Frameworks for Programming RNA Devices (Maung Nyan Win, Joe C. Liang and Christina D. Smolke).

16 RNA as a Catalyst: The Diels-Alderase Ribozyme (Andres Jaschke).

17 Evolving an Understanding of RNA Function by In Vitro Approaches (Qing Wang and Peter J. Unrau).

18 The Chemical Biology of Aptamers: Synthesis and Applications (Gunter Mayer and Bernhard Wulffen).

19 Nucleic Acids as Detection Tools (Jeffrey C.F. Lam, Sergio Aguirre and Yingfu Li).

20 Bacterial Riboswitch Discovery and Analysis (Tyler D. Ames and Ronald R. Breaker).

Index.