1. Edition - July 2009
87.90 Euro
2009. XI, 195 Pages, Hardcover
65 Fig. (7 Colored Fig.), 8 Tab. 
- Practical Approach Book -
ISBN-10: 3-527-32116-0
ISBN-13: 978-3-527-32116-2 - Wiley-VCH, Weinheim






Content

Sample Chapter

Index



Short description
Current methods for the separation and purification of RNA are explained, addressing both fundamental principles and practical aspects. The complete know-how to develop and optimise laboratory protocols for the handling of every RNA species, from the very small to the very large.

From the contents
RNA EXTRACTION, SEPARATION AND ANALYSIS
The need to be able to extract, manipulate and analyze RNA
Using chemical tools to solve the problem of analysis of biological problems
The principle of chromatography and solid phase extraction
RNA Chromatography
Enzymatic treatment of RNA and analysis
Content and organization of this book

BIOLOGICAL AND CHEMICAL RNA
Why Classify RNA with biology and chemistry?
Prokaryote cellular RNA
Prokaryote sample type
Eukaryote cellular RNA
Eukaryote sample type
Other Samples (Virus, Soil, Rock)
Synthetic RNA

RNA SEPARATION - SUBSTRATES, FUNCTIONAL GROUPS, MECHANISMS AND CONTROL
Solid phase interaction
The solid phase substrate and attachment of functional groups
Reverse phase ion pairing separation mechanism
Ion exchange separation mechanism
Chaotropic denaturing interaction mechanism
Hybridization / SELEX
Gel Filtration

RNA EXTRACTION AND ANALYSIS
Transcription
Translation
Gene regulation
Use of siRNA to investigate gene function

RNA CHROMATOGRAPHY
Development of RNA Chromatography
RNA Chromatography instrumentation
RNA Chromatography conditions
Temperature modes of RNA Chromatography
Comparison of gel electrophoresis and liquid chromatography
Analysis of human telomerase RNA under non denaturing conditions

RNA CHROMATOGRAPHY, SEPARATION AND ANALYSIS
Features of RNA Chromatography
Separation of double-stranded and single-stranded RNA
Separation of cellular RNA species
Separation of messenger RNA from total and ribosomal RNA
Analysis of transfer RNA
Chromatography and analysis of synthetic oligo ribonucleotides
Application of RNA and DNA Chromatography in cDNA library synthesis
Chromatography analyses of RT-PCR and competitive RT-PCR products
Alternative splicing
Differential messenger RNA display via RNA Chromatography

RNA STRUCTURE-FUNCTION PROBING
Definition of the Structure-function paradigm
Structure determination of RNA
Footprinting, model building and functional investigations

APPENDIX
Chromatographic Separation Equotations and Priciples for RNA Separation
HPLC Instrumentation and Operation
RNA Chromatographic System Cleaning and Passivation Treatment