Short description A revolutionary text on RNA biology that captures all the excitement of this booming field. From ribozymes to RNA interference to RNomics - everything is competently explained and put into perspective.
From the contents Preface
PART I: nRNA
INTRODUCTION RNA Building Blocks RNA Folding The RNA World Hypothesis Functions of RNA Protein Classes that are Required for RNA Function TRANSCRIPTION OF PRE-mRNAs Structure and Organization of Protein Coding Genes Transcription of mRNAs by RNA Polymerase II Transcriptional Termination of Pre-mRNAs Transcription is Coupled to Other mRNA Maturation Steps Summary CAPPING OF THE PRE-mRNA 5' END m7 G-cap Structure mRNA Capping Enzymes 5' Capping is Coupled to Transcription 5' Cap Binding Proteins Summary 3' END PROCESSING OF PRE-mRNAs Polyadenylation Signals Proteins Involved in 3' End Processing of Pre-mRNAs 3' End Processing is Tightly Linked to Transcriptional Termination Alternative Polyadenylation Cytoplasmic Polyadenylation 3' End Processing of Histone mRNAs Summary SPLICING OF EUKARYOTIC PRE-mRNAS Group I, II and III Introns The Mechanism of pre-mRNA Splicing The Spliceosome The U12-Dependent Minor Spliceosome Coupling of Splicing with Transcription and 5' Capping Alternative Splicing and the Complexity of Genomes Summary Questions mRNA EXPORT FROM THE NUCLEOUS TO THE CYTOPLASM Nuclear Import and Nuclear Export mRNA Export Receptors Adaptors the Bridge mRNAs with Export Receptors Mechanism of mRNA Export Coupling of mRNP Export to Other Steps of mRNA Maturation Summary Questions TRANSLATION Amino Acids, mRNAs, tRNAs THe Ribosome The Mechanisms of Translation Translational Regulation Coupling Translation with Other mRNA Maturation and Quality Control Steps Summary Questions DEADENYLATION OF mRNA Deadenylating Enzymes Summary Questions mRNA DECAPPING Decapping Enzymes are the Core of the mRNA Decapping Machinery Scavenger Decapping Enzyme DcpS Regulation of mRNA Decapping Intracellular Localization of mRNA Decapping Summary Questions mRNA DECAY PATHWAYS Deadenylation-Dependent mRNA Decay Deadenylation-Independent mRNA Decay Endoribonuclease-Mediated mRNA Decay Regulation of mRNA Decay RNA Degradation in Bacteria Summary Questions mRNA QUALITY CONTROL Nuclear mRNA Quality Control Mechanisms Nonsense-Mediated mRNA Decay (NMD) Other nRNA Quality Control Pathways Summary Questions
PART II: Non-Coding RNA Biology
RIBOSOMAL RNAS AND THE BIOGENESIS OF RIBOSOMES Genomic Organization of Ribosomal RNA Genes Transcription of Ribosomal RNA Genes Maturation of rRNAs Nuclear Export of Ribosomal Subunits Modification, Structure and Fucntion of rRNAs Summary Questions TRANSFER RNAS Genomic Organization and Transcription of tRNA Genes Processing to Mature tRNAs tRNA Modifications Nuclear Export of tRNAs Tertiary Structure of tRNAs Summary THE 7SL RNA AND THE SIGNAL RECOGNITION PARTICLE Architecture of the SRP SRP-Mediated Protein Translocation Summary REGULATION OF TRANSCRIPTION: THE 7SK SMALL NUCLEAR RNA Architecture of the 7SK snRNA The 7SK snRNP Functions as Transcriptional Regulator Other Small Non-Coding RNAs that Interfere with Transcription Summary SMALL NUCLEOLAR RNAS Genomic Organization and snoRNA Transcription Box H/ACA snoRNAs Box C/D snoRNAs Maturation of Functional snoRNPs Orphan snoRNAs The Telomerase RNP Summary SPLICEOSOMAL SMALL NUCLEAR RNAS Transcription and Maturation of Spliceosomal snRNAs The Structure of UsnRNPs Assembly of Spliceosomal snRNPs Summary Questions SMALL NON-CODING RNAS AND THE MECHANISM OF GENE SILENCING Short Interfering RNAs and the Mechanism of RNA Interference Dicer RNA-Dependent RNA Polymerases Argonaute Proteins microRNAs and the Regulation of Gene Expression PiRNAs and the Regulation of Mobile Genetic Elements in the Germ Line Small RNAs with Functions in Chromatin Regulation The CRISPR System - A Bacterial and Archaeal Defense Mechanism Summary LONG NON-CODING RNAS The XIST Non-Coding RNA and X Chromosome Inactivation Dosage Compensation n Flies Non-Coding RNAs and the Regulation of Imprinting The Regulation of HOX Genes by Long Non-Coding RNAs Long Non-Coding RNAs are Common in Complex Genomes Summary RNA EDITING RNA Editing by U Insertions or Deletions RNA Editing by Base Modification Summary RIBOZYMES - CATALYTIC RNA MOLECULES Identification of Catalytic RNAs Mechanisms and Secondary Structures of Different Ribozymes Summary RIBOSWITCHES AND RNA SENSORS Mechanisms of Riboswitch Function Riboswitch Structures RNA Thermometers Summary RNOMICS "Omics" Approaches Experimental RNA Profiling Strategies RNA Biology and the Complexity of Genomes Summary APPENDIX Answers to Questions