|Cleland, Jeffrey L. / Craik, Charles S. (eds.)|
Principles and Practice
1. Edition March 1996
1996. 544 Pages, Hardcover
ISBN 978-0-471-10354-7 - John Wiley & Sons
This provides a thorough introduction to protein biophysics via chapters dealing with experimental and theoretical determination of protein structure. It discuss each of the various expressions systems currently available and explore the relative advantages and limitations of each system, it also cover the difficulty in achieving correctly folded and functional proteins, the relationship between the structure and function of proteins, and specific examples of the use of protein engineering in construction of synthetic metal binding sites in proteins, the manufacture of tissue plasminogen activatior, and the generation of therapeutic antibodies.
From the contents
Partial table of contents:
Protein Conformation (F. Cohen & D. Hearst).
Predicting the Conformation of Proteins from Sequence Data (S. Benner).
Expression of Proteins in Bacteria (G. Georgiou).
Expression of Heterologous Gene Products in Yeast (S. Pichuantes, et al.).
Insect Cell Expression Technology (V. Luckow).
Protein Expression in Xenopus Oocytes (M. Marino).
Protein Engineering for Stability (S. Braxton).
Structure-Function Relationships for Protein Design (C. Gibbs).
Bacteriophage Display Libraries (J. Winter).
Engineering Therapeutic Antibodies (R. Kelley).
Site-Directed Drug Design (D. DeCamp, et al.).