1. Edition - May 1998
209.- Euro
1998. 540 Pages, Softcover
ISBN-10: 0-471-17279-0
ISBN-13: 978-0-471-17279-6 - John Wiley & Sons








Short description
This book helps investigators understand the basic concepts and practical concerns associated with the use of antisense oligonucleotides to modify gene expression. It provides a balanced overview of the field from a very practical standpoint and describes this cutting-edge technology that has been embraced because of its potential therapeutic uses in clinical conditions ranging from cancers to infectious diseases.

From the contents
CHEMISTRY, OLD AND NEW.

Oligonucleoside Methylphosphonates: Synthesis and Properties (P. Miller).

Oligo(Nucleoside Phosphorothioate)s (P. Guga, et al.).

Modified Oligodeoxynucleotides as Antisense Therapeutics (P. Seeberger & M. Caruthers).

Novel Chemistry (K. Altmann, et al.).

OLIGONUCLEOTIDE INTERNALIZATION, MECHANISM OF ACTION, AND NON-SEQUENCE SPECIFICITY.

Cellular Uptake and Biodistribution of Oligodeoxynucleotides (B. Hanss, et al.).

Use of Cationic Lipid Complexes for Antisense Oligonucleotide Delivery (C. Bennett).

Nonantisense Effects of Antisense Oligonucleotides (L. Neckers & K. Iyer).

Ribonuclease H-Mediated Antisense Effects of Oligonucleotides and Controls for Antisense Experiments (D. Tidd).

SEQUENCE-SPECIFIC INHIBITION OF GENE EXPRESSION.

The Development of C-5 Propyne Oligonucleotides as Inhibitors of Gene Function (W. Flanagan & R. Wagner).

The Use of Antisense Oligonucleotides to Inhibit Expression of Isozymes of Protein Kinase C (N. Dean, et al.).

BCR-ABL as a Target for Antisense Intervention (S. O'Brien & T. Smetsers).

The NF-kB Transcription Factor (R. Narayanan).

Disruption of the Map Kinase Signaling Pathway Using Antisense Oligonucleotide Inhibitors Targeted to RAS and RAF Kinase (B. Monia).

Protein Kinase A-Directed Antisense Blockade of Cancer Growth: Single Gene-Based Therapeutic Approach (Y. Cho-Chung).

Use of Antisense Oligonucleotides in the Central Nervous System: Why Such Success?

(M. McCarthy).

APPLIED ANTISENSE OLIGONUCLEOTIDE THERAPEUTICS.

Perturbing Hematopoietic Cell Gene Expression with Oligodeoxynucleotides: Research and Clinical Applications (A. Gewirtz & M. Ratajczak).

BCL2 (B. Jansen & B. Brown).

Biological Activity of Guanosine Quartet-Forming Oligonucleotides (R. Rando & M. Hogan).

OLIGONUCLEOTIDES AS ANTI-HIV AGENTS.

Perspectives on Antisense Technology Against HIV (J. Gee, et al.).

In Vivo Pharmacokinetics of Oligonucleotides (S. Agrawal).

Early Clinical Trails with Gem 91, A Systemic Oligodeoxynucleotide (R. Martin).

THERAPEUTIC OLIGONUCLEOTIDE DATA BASE: PHARMACOKINETICS, IMMUNE STIMULATION, AND USE AS ANTIRESTENOTIC AGENTS.

Antisense Therapy to Inhibit Angioplasty Restenosis (L. Rabbani & W. Wang).

Pharmacokinetics of Oligonucleotides: A Review of Current Knowledge and Issues for the Future (T. Wallace & P. Cossum).

Leukocyte Stimulation by Oligodeoxynucleotides (A. Krieg).

SELECTED OLIGODEOXYNUCLEOTIDE DEVELOPMENTAL TOPICS: SPLICING AND TRIPLEXES.

Modification of Alternative Splicing of Pre-mRNA by Antisense Oligonucleotides (R. Kole).

Gene-Targeted Triple-Helix-Forming Oligonucleotides (F. Svinarchuk & C. Malvy).

Triplex-Forming Oligonucleotides for Genetic Manipulation: An Alternative View (A. Faruqi & P. Glazer).

A REVIEW OF RIBOZYME TECHNOLOGY.

Therapeutic Ribozymes: Principles, Applications, and Problems (J. Rossi).

Index.