Short description This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by: * a section on difference gel electrophoresis * the chapter on proteome analysis is practically all new and considerably extended * around 10 % new literature references.
From the contents PART I: FUNDAMENTALS Introduction Electrophoresis Isotachophoresis Isoelectric Focusing Blotting Interpretation of Electropherograms Proteome Analysis Instrumentation PART II: EQUIPMENT AND METHODS Equipment for Part II Method 1: PAGE of Dyes Method 2: Agarose and Immuno Electrophoresis Method 3: Titration Curve Analysis Method 4: Native PAGE in Amphoteric Buffers Method 5: Agarose IEF Method 6: PAGIEF in Rehydrated Gels Method 7: Horizontal SDS-PAGE Method 8: Vertical PAGE Method 9: Semi-dry Blotting of Proteins Method 10: IEF in Immobilized pH Gradients Method 11: High-resolution 2D Electrophoresis Method 12: PAGE of Double Stranded DNA Method 13: Native PAGE of Single Stranded DNA Method 14: Denaturing Gradient Gel Electrophoresis Method 15: Denaturing PAGE of DNA Appendix A: Trouble-shooting References Index