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Westermeier, Reiner
Electrophoresis in Practice
A Guide to Methods and Applications of DNA and Protein Separations

4., revised and enlarged Edition - October 2004
109.- Euro
2004. XX, 406 Pages, Hardcover
191 Fig. (10 Colored Fig.), 34 Tab. 
- Textbook -
ISBN-10: 3-527-31181-5
ISBN-13: 978-3-527-31181-1 - Wiley-VCH, Weinheim


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Short description
This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by:
* a section on difference gel electrophoresis
* the chapter on proteome analysis is practically all new and considerably extended
* around 10 % new literature references.

From the contents
PART I: FUNDAMENTALS
Introduction
Electrophoresis
Isotachophoresis
Isoelectric Focusing
Blotting
Interpretation of Electropherograms
Proteome Analysis
Instrumentation
PART II: EQUIPMENT AND METHODS
Equipment for Part II
Method 1: PAGE of Dyes
Method 2: Agarose and Immuno Electrophoresis
Method 3: Titration Curve Analysis
Method 4: Native PAGE in Amphoteric Buffers
Method 5: Agarose IEF
Method 6: PAGIEF in Rehydrated Gels
Method 7: Horizontal SDS-PAGE
Method 8: Vertical PAGE
Method 9: Semi-dry Blotting of Proteins
Method 10: IEF in Immobilized pH Gradients
Method 11: High-resolution 2D Electrophoresis
Method 12: PAGE of Double Stranded DNA
Method 13: Native PAGE of Single Stranded DNA
Method 14: Denaturing Gradient Gel Electrophoresis
Method 15: Denaturing PAGE of DNA
Appendix A: Trouble-shooting
References
Index


 
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