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RNA, RNA interference, ribozyme switches, microRNA, structural studies, aptamers, and more ...
The importance of RNA interference was recognized by the Nobel Assembly, who awarded the 2006 Nobel Prize in Physiology or Medicine jointly to Andrew Z. Fire (Stanford School of Medicine, USA) and Craig C. Mello (University of Massachusetts, USA) for their discovery of RNA interference—gene silencing by double-stranded RNA.
Thomas Tuschl, winner of the Wiley Prize in Biomedical Sciences in 2003, discussed RNA Interference and Small Interfering RNAs in a Minireview in ChemBioChem, 2001, 2, 239.
Find all articles on RNA or just on RNA interference in Wiley Online Library...
Timur R. Samatov, Alexander Wolf, Peter Odenwälder, Sergey Bessonov, Céline Deraeve, Robin S. Bon, Herbert Waldmann, Reinhard Lührmann
Psoromic Acid Derivatives: A New Family of Small-Molecule Pre-mRNA Splicing Inhibitors Discovered by a Stage-Specific High-Throughput in Vitro Splicing Assay [Communication]
Snapshots of spliceosome assembly: A high-throughput in vitro splicing assay has been developed for screening a compound library. The discovered family of pre-mRNA splicing inhibitors comprises lichen secondary metabolites that allow the enrichment of the spliceosomal complexes formed after the spliceosome activation step. Here we identify the structural features important for the compounds' inhibitory activity.
Robert J. Mart, Piotr Wysoczański, Sabine Kneissl, Antonio Ricci, Andrea Brancale, Rudolf K. Allemann
Design of Photocontrolled RNA-Binding Peptidomimetics [Communication]
Positively constrained: The first examples of photocontrolled RNA binding peptides are described. The large number of positively charged sides chains in the Rev response element (RRE) of an HIV type I targeting α-helix imposes constraints on the choice of azobenzene-derived crosslinker.
Marie Renault, Shane Pawsey, Martine P. Bos, Eline J. Koers, Deepak Nand, Ria Tommassen-van Boxtel, Melanie Rosay, Jan Tommassen, Werner E. Maas, Marc Baldus
Solid-State NMR Spectroscopy on Cellular Preparations Enhanced by Dynamic Nuclear Polarization [Communication]
A peek inside: Dynamic nuclear polarization (DNP) enhances the spectroscopic sensitivity of solid-state NMR measurements of uniformly (13C,15N)- labeled preparations of Escherichia coli cells by more than an order of magnitude (see picture; MW=microwaves, ε=enhancement factor). The major molecular components in the cells can be characterized in this way.
Angew. Chem. Int. Ed., February 1, 2012, DOI: 10.1002/anie.201105984
Sandip A. Shelke, Snorri Th. Sigurdsson
Site-Directed Spin Labelling of Nucleic Acids [Microreview]
Debojit Bose, Gopal Jayaraj, Hemant Suryawanshi, Prachi Agarwala, Subrata Kumar Pore, Rajkumar Banerjee, Souvik Maiti
The Tuberculosis Drug Streptomycin as a Potential Cancer Therapeutic: Inhibition of miR-21 Function by Directly Targeting Its Precursor [Communication]
No dice: MicroRNAs (miRNAs) fine-tune gene expression, deregulation of which has been causally associated with a number of debilitating conditions. Streptomycin, a well-known aminoglycoside drug, binds to RNA secondary structures and is shown to inhibit miR-21 function by direct binding to its precursor, thus presumably interfering with the processing by the Dicer enzyme (see scheme).
Hiroshi Ito, Masaaki Urushihara, Xingguo Liang, Hiroyuki Asanuma
Improvement of RNAi Activity and Strand Selectivity of RISC Formation by Modified siRNA Involving Intercalators near 5' Termini [Communication]
RISC avoidance: By introducing an intercalator such as azobenzene into the 5'-end of the sense strand at a D-threoninol linker, RNAi activity was greatly improved. This enhancement of RNAi activity was attributed to selective loading of the antisense strand into RISC and the suppression of RISC assembly with the modified sense strand.
Laurence Delaurière, Zeyuan Dong, Katta Laxmi-Reddy, Frédéric Godde, Jean-Jacques Toulmé, Ivan Huc
Deciphering Aromatic Oligoamide Foldamer–DNA Interactions [Communication]
Finest selection: Side-chain selective, end-group selective, diastereoselective, and RNA- vs. DNA-selective interactions have been revealed between multiturn helical aromatic amide foldamers having cationic side chains and G-quadruplex aptamers.
Heidi Korhonen, Satu Mikkola, Nicholas H. Williams
The Mechanism of Cleavage and Isomerisation of RNA Promoted by an Efficient Dinuclear Zn2+ Complex [Full Paper]
The missing zinc: A dinuclear ZnII complex produced a million-fold acceleration of RNA cleavage at pH 6.5 and 25 °C. Remarkably, the cooperative effects of the Zn ions and ligand-based hydrogen-bond-donating groups stabilise a phosphorane intermediate sufficiently for it to isomerise as well, with cleavage enhanced selectively through general acid catalysis.
Günter Mayer, Sabine Lennarz, Falk Rohrbach, Fabian Tolle
Plug and Play with RNA [Highlight]
Retooling RNA: RNA aptamers are high-affinity ligands that can be assembled with other structures to yield multivalent molecules. These properties have been addressed in two recent studies: One describes a GFP-like RNA reporter used to study the dynamics of endogenous RNA; the other study reports on an aptamer-templated assembly of multi-enzyme complexes in bacteria for the controlled production of secondary molecules (see picture).
Niles Lehman, Eric J. Hayden
Template-Directed RNA Polymerization: The Taming of the Milieu [Highlight]
No-bias binding: The abiotic template-directed synthesis of RNA could have been a key process in the origins of life on Earth. Recreating this process in the laboratory has been challenging, yet a combination of strategies has given rise to a synthesis that is both efficient and unbiased against any of the four nucleotides.
Norbert Polacek
The Ribosome Meets Synthetic Biology [Highlight]
A recent advance in custom-designed, cell-free translation systems that makes use of purified and recombinant components opens up new perspectives for synthesizing non-natural translation products. Combining these systems with chemically engineered ribosomes sets the stage for novel synthetic biology projects.
Jens Tilsner, Cristina Flors
FIT for Purpose: PNA-Based Probes Enable mRNA Imaging in Living Cells [Highlight]
If the dye FITs: A new approach for imaging mRNA in vivo is based on forced intercalation (FIT) of a dye that substitutes a base of a peptide nucleic acid hybridisation probe. Upon hybridisation with complementary mRNA, the fluorescence of the dye is greatly enhanced, improving the signal-to-noise ratio for fluorescence microscopy in living cells.
© Wiley-VCH 2011.
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