Focusing on the Future
RMS - Royal Microscopical Society
1. Auflage November 2019
248 Seiten, Hardcover
Wiley & Sons Ltd
Preis: 115,00 €
Preis inkl. MwSt, zzgl. Versand
Brings a fresh point of view to the current state of correlative imaging and the future of the field This book provides contributions from international experts on correlative imaging, describing their vision of future developments in the field based on where it is today. Starting with a brief historical overview of how the field evolved, it presents the latest developments in microscopy that facilitate the correlative workflow. It also discusses the need for an ideal correlative probe, applications in proteomic and elemental analysis, interpretation methods, and how correlative imaging can incorporate force microscopy, soft x-ray tomography, and volume electron microscopy techniques. Work on placing individual molecules within cells is also featured.
Correlative Imaging: Focusing on the Future offers in-depth chapters on: correlative imaging from an LM perspective; the importance of sample processing for correlative imaging; correlative light and volume EM; correlation with scanning probe microscopies; and integrated microscopy. It looks at: cryo-correlative microscopy; correlative cryo soft X-ray imaging; and array tomography. Hydrated-state correlative imaging in vacuo, correlating data from different imaging modalities, and big data in correlative imaging are also considered.
* Brings a fresh view to one of the hottest topics within the imaging community: the correlative imaging field
* Discusses current research and offers expert thoughts on the field's future developments
* Presented by internationally-recognized editors and contributors with extensive experience in research and applications
* Of interest to scientists working in the fields of imaging, structural biology, cell biology, developmental biology, neurobiology, cancer biology, infection and immunity, biomaterials and biomedicine
* Part of the Wiley-Royal Microscopical Society series
Correlative Imaging: Focusing on the Future will appeal to those working in the expanding field of the biosciences, correlative microscopy and related microscopic areas. It will also benefit graduate students working in microscopy, as well as anyone working in the microscopy imaging field in biomedical research.
Chapter 1: It's a small, small world: a brief history of biological correlative microscopy
Chapter 2: Challenges for CLEM from a Light Microscopy Perspective"
Chapter 3: The importance of sample processing for correlative imaging (or, rubbish in, rubbish out)
Chapter 4: 3D CLEM: Correlating Volume Light and Electron Microscopy
Chapter 5: Can correlative microscopy ever be easy? An array tomography viewpoint
Chapter 6: Correlative microscopy using scanning probe microscopes
Chapter 7: Future of CLEM - Integrated Light and Electron Microscopy
Chapter 8: Cryo-correlative light and electron microscopy: Towards in situ structural biology
Chapter 9: Correlative cryo soft X-ray imaging.
Chapter 10: Correlative light- and liquid-phase scanning transmission electron microscopy for studies of protein function in whole cells
Chapter 11: Correlating data from different imaging modalities
Chapter 12: Big data in correlative imaging
Chapter 13: The Future of CLEM: Summary
DR LUCY COLLINSON, PHD, has been working in the field of Correlative Microscopy for the last 18 years and is currently Head of Electron Microscopy at the Francis Crick Institute, London, United Kingdom.