1. Edition - November 2002
142.- Euro
2002. XVIII, 484 Pages, Hardcover
197 Fig. (9 Colored Fig.), 24 Tab. 
- Monograph -
ISBN-10: 3-527-30465-7
ISBN-13: 978-3-527-30465-3 - Wiley-VCH, Weinheim









Short description
The great importance of BioNMR has just recently been recognized with the Nobel Prize in Chemistry, and indeed the editor of this book worked alongside the Laureate, Kurt Wüthrich.
Written by authors active in the development of new drugs for leading pharmaceutical companies, the text offers a practical, hands-on approach to the theory and guidance needed to use NMR spectroscopy for examining the binding of an active substance to its target protein. With its successful mixture of core information and application strategies, coupled with real-life examples, this is an invaluable guide for both NMR spectroscopists and pharmaceutical researchers.



From the contents
BASIC TECHNIQUES
Modern Methods for Expression of Proteins in Isotopically Enriched Form
Structure Calculation Using Automated Techniques
Achieving Better Sensitivity, Less Noise and Less Artifacts in NMR Spectra

NMR OF BIOMOLECULES
NMR Strategies for Protein Assignment
NMR of Membrane-Associated Peptides and Proteins
NMR of Nucleic Acids

MODERN SPECTROSCOPIC TECHNIQUES
Methods for the Measurement of Angle Restraints from Scalar and Dipolar Couplings as well as from Cross Correlated Relaxation: Application to Biomolecules
Orientational Restraints
Scalar Coupling Across Hydrogen Bonds
TROSY:Transverse Relaxation-Optimized Spectroscopy
MAS Solid-State NMR of Isotopically Enriched Biological Samples
Determination of Protein Dynamics Using 15N Relaxation Measurements

TOOLS FOR INVESTIGATION OF DRUG-RECEPTOR COMPLEXES AND FOR LIGAND SCREENING
The Determination of Equilibrium Dissociation Constants of Protein-Ligand Complexes by NMR
Experiments in NMR-based Screening
The Use of Spin-Labels in NMR-supported Lead Finding and Optimization
NMR of Weakly Binding Ligands
Isotope Filter and Editing Techniques

STRATEGIES FOR DRUG DEVELOPMENT USING NMR
Strategies for NMR Screening and Library Design
Strategies for Hit Finding Using NMR
Strategies for Drug Discovery Using NMR
NMR-based Drug Design: Approaches for Very Large Proteins