1. Edition - October 2008
83.90 Euro
2008. 272 Pages, Hardcover
- Practical Approach Book -
ISBN-10: 0-470-25886-1
ISBN-13: 978-0-470-25886-6 - John Wiley & Sons






Sample Chapter

Short description
This reference presents a wide variety of mass spectrometry methods used to explore structural mechanisms, protein dynamics and interactions between proteins. Preliminary chapters cover mass spectrometry methods for examining proteins and are then followed by chapters devoted to presenting very practical, how-to methods in a detailed way. Methods include deuterium exchange, covalent labeling, footprinting, plistex, and computational approaches. The coverage of footprinting and plistex sets the book apart from the competition.

From the contents
Foreword.

Contributors.

1. Overview of Mass Spectrometry Technologies for Examining Protein Structure: Current and Future Directions (Shannon M. Swiatkowski and Mark R. Chance).

References.

2. Hydrogen Exchange Mass Spectrometry: Principles and Capabilities (Sebastien Brier and John R. Engen).

Acknowledgment.

References.

3. Covalent Labeling Methods for Examining Protein Structure and Protein Interactions (Keiji Takamoto and Janna Kiselar).

References.

4. Complementary Methods for Structure Determination: Hydroxyl-Radical-Mediated Footprinting and Deuterium Exchange Mass Spectrometry as Applied to Serpin Structure (Xiaojing Zheng and Patrick L. Wintrode).

Acknowledgement.

References.

5. Deuterium Exchange Approaches for Examining Protein Interactions: Case Studies of Complex Formation (Elizabeth A. Komives).

References.

6. Hydrogen/Deuterium Exchange Studies of Viruses (Sebyung Kang and Peter E. Prevelige Jr.).

References.

7. Use of Enhanced Peptide Amide Hydrogen/Deuterium Exchange-Mass Spectrometry (DXMS) in the Examination of Protein-Protein Interactions (Yoshitomo Hamuro, Stephen J. Coales, Lora L. Hamuro, and Virgil L. Woods Jr.).

Abbreviations.

Acknowledgements.

References.

8. Cross-linking as a Tool to Examine Protein Complexes: Examples of Cross-linking Strategies and Computational Modeling (Evgeniy V. Petrotchenko and Christoph H. Borchers).

References.

9. Complex Formation in the Actin Cytoskeleton: Cross-linking Tools to Define Actin Protein Structure and Interactions (Sabrina Benchaar and Emil Reisler).

Acknowledgement.

References.

10. Computational Approaches to Examining Protein-Protein Interactions: Combining Experimental and Computational Data in the Era of Structural Genomics (J.K. Amisha Kamal).

Acknowledgment.

References.

11. Studies of Intact Proteins and Protein Complexes: ESI MS Approaches (Igor A. Kaltashov, Rinat R. Abzalimov, Agya K. Frimpong, and Stephen J. Eyles).

Acknowledgments.

References.

12. Two Approaches to Mass Spectrometric Protein Footprinting: PLIMSTEX and FPOP (Michael L. Gross, Mei M. Zhu, and David M. Hambly).

Abbreviations.

Acknowledgments.

References.

Index.