Short description This first practical reference on sample preparation addresses a key bottleneck in proteomics studies. Includes step-by-step protocols adaptable to any specific proteome analysis task.
From the contents PERSPECTIVES IN PROTEOMICS SAMPLE PREPARATION Introduction General Aspects of Sample Preparation for Comprehensive Proteome Analysis Proteomics: A Philosophical Perspective MASS SPECTROMETRY A Practical Guideline to Electrospray Ionization Mass Spectrometry for Proteomics Application Sample Preparation for the Application of MALDI Mass Spectrometry Sample Preparation for Label-Free Proteomic Analyses of Body Fluids by Fourier Transform Ion Cyclotron Mass Spectrometry Sample Preparation for Differential Proteome Analysis: Labelling Technologies for Mass Spectrometr Determining Protein Localization within Subcellular Compartments using Stable IsotopeTagging ELECTROPHORESIS Sample Preparation for Two-Dimensional Gel Electrophoresis Sample Preparation for Native Electrophoresis Sample Preparation for LC-MS/MS using Free Flow Electrophoresis Sample Preparation for Proteomics OPTICAL METHODS High-Throughout Proteomics: Spinning Disc Interferometry (SDI) Optical Proteomics on Cell Arrays Sample Preparation by Laser Microdissection and Catapulting for Proteome Analysis Sample Preparation for Flow Cytometry CHROMATOGRAPHY Sample Preparation for HPLC-Based Proteome Analysis Sample Preparation for Two-Dimensional Phosphopeptide Mapping and Phosphoamino Acid Analysis STRUCTURAL PROTEOMICS Exploring Protein-Ligand Interactions by Solution NMR Sample Preparation for Crystallography INTERACTION ANALYSIS Sample Preparation for Protein Complex Analysis by the Tandem Affinity Purification (TAP) Methods Exploring Membrane Proteomes POST-TRANSLATIONAL MODIFICATIONS Sample Preparation for Phosphoproteome Analysis Sample Preparation for Analysis of Post-Translational Modifications: Glycosylation SPECIES-DEPENDENT PROTEOMICS Sample Preparation and Data Processing in Plant Proteomics Sample Preparation for MudPIT with Bacterial Protein Samples Sample Preparation for the Cell-Wall Proteome Analysis of Yeast and Fungi THE HUMAN PROTEOSOME Clinical Proteomics: Sample Preparation and Standardization Stem Cell Proteomics BIOINFORMATICS Bioinformatics Support for Mass Spectrometric Quality Control Use of Physio-Chemical Properties in Peptide and Protein Identification
