Principles and Methods
3. Auflage August 2017
XX, 316 Seiten, Hardcover
The author's integrated approach to the theoretical and methodological aspects of enzyme kinetics makes this a highly useful tool for understanding, measuring and modeling enzyme action as well as an excellent introduction to the field.
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Now in full color for a more intuitive learning experience, this new edition of the long-selling reference also features a number of new developments in methodology and the application of enzyme kinetics.
Starting with a description of ligand binding equilibria, the experienced author goes on to discuss simple and complex enzyme reactions in kinetic terms. Special cases such as membrane-bound and immobilized enzymes are considered, as is the influence of external conditions, such as temperature and pH value. The final part of the book then covers a range of widely used measurement methods and compares their performance and scope of application.
With its unique mix of theory and practical advice, this is an invaluable aid for teaching as well as for experimental work.
Interaction between macromolecules and ligands
Identical independent binding sites
Non-identical independent binding sites
Identical interacting binding sites, cooperativity
Non-identical interacting binding sites
PART II: Enzyme Kinetics
Steady-state kinetics and the Michaelis-Menten Equation
Reversible enzyme reactions
Rate equations of complex enzyme mechanisms
pH and temperature dependence
Isotope exchange kinetics and isotope effects
Enyzmes with polymeric substrates
Kinetics of transport processes
Enzyme reactions at membrane interfaces
PART III: Methods
Methods for investigating multiple equilibria
Measurement of fast reactions