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First there were Southern blots for DNA, named after the inventor, then there were jokingly named Northern blots for RNA and Western blots for proteins. Here we have Southwestern blots (not a spotted airline), a combination of blotted protein targets and 32P-labeled DNA probes. Jiang et al. used a number of tools, including oligonucleotide trapping, 2-DE, Southwestern blots, an Electrophoretic Mobility Shift Assay (EMSA) and an antibody-enhanced shift assay (“supershift”) in their search for the transcription factors that control the expression of telomerase and its associated reverse transcriptase (“hTERT”). The targets of choice are a proximal and a distal E-box sequence with the promoter in between. Using LC-nESI-MS/MS on isolated spots revealed several factors, particularly interesting was Upstream Stimulatory Factor 2 (USF 2).

To appear in PROTEOMICS Issue 02.

Purification and identification of a transcription factor, USF-2, binding to E-box element in the promoter of human telomerase reverse transcriptase (hTERT).

Jiang, S. et al., Proteomics 2010, 10, DOI: 10.1002/pmic.200800693