Carrier-bound Immobilized Enzymes
Principles, Application and Design
1. Auflage September 2005
XV, 563 Seiten, Hardcover
248 Abbildungen
67 Tabellen
Handbuch/Nachschlagewerk
Kurzbeschreibung
The authoritative reference for the developer and user of carrier-bound enzymes covers all types of enzyme immobilization methods and supports, including novel functional supports and new strategies, such as enzyme entrapment and encapsulation.
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The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes.
A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work.
Introduction
The Past
Implications from the Past
Prospective and Future Development
ADSORPTION-BASED IMMOBILIZATION
Introduction and Classification
Principles of Adsorptive Enzyme Immobilization
Requirements of the Carriers
Factors which Dictate Enzyme Performance
Preparation of Immobilized Enzymes by Adsorption
COVALENT ENZYME IMMOBILIZATION
Introduction
Physical Nature of Carriers
Chemical Nature of Carriers
Amino Acid Residues for Covalent Binding of Enzymes
Factors Influencing Enzyme Performance
Preparation of Active Carriers
ENZYME ENTRAPMENT
Introduction and Definition
Requirements of the Carriers
Effect of the Entrapment
Preparation of Entrapped Enzymes
ENZYME ENCAPSULATION
Introduction and Classification
Effect of Encapsulation
Preparation of Encapsulated Enzymes
NON-CONVENTIONAL ENZYME IMMOBILIZATION
Coating-based Immobilization
Site-specific Immobilization
Immobilization in Organic Solvents
Imprinting Immobilization
Modification-based Immobilization
Post-immobilization Techniques
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