1. Auflage - Januar 2010
159,- Euro
2010. XXV, 435 Seiten, Hardcover
98 Abb. (59 Farbabb.), 17 Tab. 
- Handbuch/Nachschlagewerk -
ISBN-10: 3-527-32604-9
ISBN-13: 978-3-527-32604-4 - Wiley-VCH, Weinheim

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Inhalt

Probekapitel

Studentenmaterial



Kurzbeschreibung
Induced mutagenesis is the most promising screening method for novel breeds of crops. This book provides a complete overview of the current techniques and the analysis of the resulting mutations. A must-have for agricultural and crop breeding scientists in industry and academia.

Aus dem Inhalt
INDUCED MUTATIONS
Physically induced mutagenesis: Ion beam mutagenesis (Magori, Tanaka, Kawaguchi)
Ds transposon mutant lines for saturation mutagenesis of the Arabidopsis genome (Kuromori, Hirayama)
Use of mutants from T-DNA insertion populations generated by high-throughput screening (Stracke, Huep, Weisshaar)
Making mutations is an active process: Examining DNA polymerase errors (Eckert, Gestl)
Tnt1 induced mutations in Medicago: Characterisation and Applications (Ratet, Cosson, Wen, Tadege, Mysore)
MUTATION DISCOVERY
Sequencing by synthesis: Mutation detection with the Illumina Genome Analyzer (Lakdawalla, Schroth)
Chemical methods for mutation detection: The chemical cleavage of mismatch (CCM) method (Tabone, Sallmann, Cotton)
Mutation detection in plants by enzymatic mismatch cleavage (Till)
Mutation scanning and genotyping in plants by high resolution DNA melting (McKinney, Nay, Koeyer, Reed, Wall, Palais, Jarret, Wittwer)
In silico methods: Mutation detection software for Sanger sequencing, fragment and genome analysis (LeVan*, Liu, Shouyong)
HIGH-THROUGHPUT SCREENING METHODS
Use of TILLING for reverse and forward genetics of rice (Terauchi)
Sequencing-based high-throughput mutation screening using KeyPointTM technology (Rigola, van Eijk)
APPLICATIONS IN PLANT BREEDING
Natural and induced mutants of barley: SNPs in genes important for breeding (Thomas, Waugh, Forster, Franckowiak)
Association mapping for the exploration of genetic diversity and identification of useful loci for plant breeding (Belo, Luck)
Using mutations in corn breeding programs (Scott, Bodnar)
Gene targeting as a precise tool for plant mutagenesis (Zobell, Reiss)
EMERGING TECHNOLOGIES
Direct single-molecule DNA sequencing:Helicos BioSciences technology and applications (Jenkins, Kahvejian)
Mutation detection by genome sequencing with universal probes (Linnarsson)
Emerging Technologies: Detection of mutations with the Pinpoint Sequencer (Ju)
Emerging Technologies: Nanopore Sequencing for Mutation Detection (Rollings, Li)

Glossary