Kurzbeschreibung Dieser Laborleitfaden beschreibt die Praxis zur Analyse von Eiweißverbindungen und schließt damit eine echte Lücke in der Literatur. Er erläutert Vorbereitung, Durchführung und Auswertungsmethoden in Abhängigkeit von den Informationen, die eine Laboruntersuchung erbringen soll.
Aus dem Inhalt PERSPECTIVES IN PROTEOMICS SAMPLE PREPARATION Introduction General Aspects of Sample Preparation for Comprehensive Proteome Analysis Proteomics: A Philosophical Perspective MASS SPECTROMETRY A Practical Guideline to Electrospray Ionization Mass Spectrometry for Proteomics Application Sample Preparation for the Application of MALDI Mass Spectrometry Sample Preparation for Label-Free Proteomic Analyses of Body Fluids by Fourier Transform Ion Cyclotron Mass Spectrometry Sample Preparation for Differential Proteome Analysis: Labelling Technologies for Mass Spectrometr Determining Protein Localization within Subcellular Compartments using Stable IsotopeTagging ELECTROPHORESIS Sample Preparation for Two-Dimensional Gel Electrophoresis Sample Preparation for Native Electrophoresis Sample Preparation for LC-MS/MS using Free Flow Electrophoresis Sample Preparation for Proteomics OPTICAL METHODS High-Throughout Proteomics: Spinning Disc Interferometry (SDI) Optical Proteomics on Cell Arrays Sample Preparation by Laser Microdissection and Catapulting for Proteome Analysis Sample Preparation for Flow Cytometry CHROMATOGRAPHY Sample Preparation for HPLC-Based Proteome Analysis Sample Preparation for Two-Dimensional Phosphopeptide Mapping and Phosphoamino Acid Analysis STRUCTURAL PROTEOMICS Exploring Protein-Ligand Interactions by Solution NMR Sample Preparation for Crystallography INTERACTION ANALYSIS Sample Preparation for Protein Complex Analysis by the Tandem Affinity Purification (TAP) Methods Exploring Membrane Proteomes POST-TRANSLATIONAL MODIFICATIONS Sample Preparation for Phosphoproteome Analysis Sample Preparation for Analysis of Post-Translational Modifications: Glycosylation SPECIES-DEPENDENT PROTEOMICS Sample Preparation and Data Processing in Plant Proteomics Sample Preparation for MudPIT with Bacterial Protein Samples Sample Preparation for the Cell-Wall Proteome Analysis of Yeast and Fungi THE HUMAN PROTEOSOME Clinical Proteomics: Sample Preparation and Standardization Stem Cell Proteomics BIOINFORMATICS Bioinformatics Support for Mass Spectrometric Quality Control Use of Physio-Chemical Properties in Peptide and Protein Identification
