|  | Westermeier, Reiner / Naven, Tom / Höpker, Hans-Rudolf
Proteomics in Practice
A Guide to Successful Experimental Design
 2., vollständig überarbeitete Auflage - März 2008
115,- Euro
2008. XX, 482 Seiten, Hardcover
159 Abb. (28 Farbabb.), 51 Tab.
- Praktikerbuch -
ISBN 978-3-527-31941-1 - Wiley-VCH, Weinheim
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Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.
Aus dem Inhalt
Preface
Foreword
Abbreviations
Glossary of terms
INTRODUCTION
History
Critical points
Hypothesis driven approach vs. High Throughput
Complementarities of workflows
Concept of experimental planning
Success stories, highlights of useful references
PART I: PROTEOMICS TECHNOLOGY
Electrophoresis
Liquid Chromatography
Mass spectrometry
Bioinformatics tools / data mining
Methods under development
PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS
Strategy where to start
Gel-based workflow
LC-based workflow
Latest trends
Validation with Western blotting
Statistic evaluation
PART III: TROUBLE SHOOTING
Gel-based workflow
LC based workflow
References
Index
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