Biology of Archaea, Volume 2
Molecular Biology of Archaea, From Genome Maintenance to the Regulation of Gene Expression
ISTE Invoiced
1. Auflage Juli 2025
256 Seiten, Hardcover
Wiley & Sons Ltd
Archaea constitute a new branch of life alongside bacteria and eukaryotes. These microorganisms are unique in their cellular and molecular aspects. They have evolutionary links with the first eukaryotic cells and are now being used to elucidate fundamental biological questions.
Champions of extremophilicity, archaea are helping to lift the veil on the limits of life on Earth. Biology of Archaea 2 presents the diverse molecular mechanisms involved in the fundamental processes of genome maintenance and regulation of gene expression in archaeal cells.
This book analyzes the complex machinery involved in chromosome replication, DNA repair, RNA synthesis (transcription) and protein synthesis (translation), and explores the different classes of RNAs and enzymes involved in RNA maturation and degradation. These regulate the stability of messenger and regulatory non-coding RNAs, and contribute to the formation of the mature forms of ribosomal RNAs and transfer RNAs. These molecular mechanisms are closely related to those of eukaryotes.
Béatrice CLOUET-D'ORVAL, Bruno FRANZETTI and Philippe OGER
Chapter 1. Replication of Archaeal Chromosomes 1
Ghislaine HENNEKE, Roxane LESTINI, Marc NADAL and Didier FLAMENT
1.1. Replication initiation 2
1.2. The elongation phase of replication 8
1.3. Okazaki fragment maturation 10
1.4. Restarting replication forks 15
1.5. Replication termination 18
1.6. Chromosome segregation and decatenation 18
1.7. References 19
Chapter 2. Archaeal DNA Repair 25
Caroline L'HERMITTE-STEAD, Anaïs BAYARD, Alexey ALEKSANDROV, Roxane LESTINI and Hannu MYLLYKALLIO
2.1. General introduction 25
2.2. Several different types of DNA damages occur in archaea 28
2.3. Different archaeal DNA repair pathways 31
2.4. Coordination of the different pathways by the replication clamp 40
2.5. Summary and conclusion 42
2.6. References 43
Chapter 3. Transcription in Archaea 45
Thomas FOUQUEAU, Duy KHANH PHUNG and Ludovic SAUGUET
3.1. RNA polymerase 47
3.2. The three stages of transcription 51
3.3. Transcription regulation 64
3.4. References 66
Chapter 4. RNA Classes and Their Maturation and Degradation Enzymes 77
Manon BATISTA, Béatrice CLOUET-D'ORVAL and Marie BOUVIER
4.1. Introduction 77
4.2. RNA classes in archaea 79
4.3. Ribonuclease families in archaea 84
4.4. Ubiquitous RNA-binding proteins in archaea 95
4.5. Conclusion 100
4.6. References 100
Chapter 5. Ribosome and Transfer RNA Biogenesis 115
Tamara BASTA and Sébastien FERREIRA-CERCA
5.1. Ribosome biogenesis 116
5.2. Synthesis and maturation of tRNAs 133
5.3. Methodical toolboxes 156
5.4. References 158
Chapter 6. The Diversity and Function of Noncoding RNAs in Archaea 167
Hubert F. BECKER and Christine GASPIN
6.1. Introduction 167
6.2. Noncoding RNA diversity 168
6.3. ncRNA identification 178
6.4. Circularization of noncoding RNAs 182
6.5. Conclusion 185
6.6. References 186
Chapter 7. Translation in Archaea 193
Emmanuelle SCHMITT and Yves MECHULAM
7.1. Initiating the translation 195
7.2. Elongation peptide synthesis 206
7.3. Translation termination and ribosome recycling 214
7.4. Conclusion 219
7.5. References 220
List of Authors 233
Index 237
Bruno Franzetti is Research Director at the CNRS, France, where he specializes in the structural biology of archaea. His research areas include biophysical and cellular mechanisms that maintain proteome integrity under extreme conditions.
Philippe Oger is Research Director at the CNRS, France. His research areas include understanding the adaptations of prokaryotes in response to extreme conditions, using a multidisciplinary approach combining the methods derived from atomic physics and cutting-edge molecular biology and modeling.
