Patch Clamping
An Introductory Guide to Patch Clamp Electrophysiology
1. Auflage Oktober 2002
186 Seiten, Softcover
Wiley & Sons Ltd
Kurzbeschreibung
Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. This comprehensive text covers the basic principles and practical applications of this important techniques. Starting with a review of the history of patch clamping, the book discusses the basic principles, platforms, equipment and environmental control and includes coverage of preparation types, recording modes, and analysis of results.
Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells.
Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results.
* This book will explain the basic principles and practical application of patch clamp electrophysiology.
* Written in a non-technical style to ensure its broad appeal to novice users
* Takes a practical approach
* This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns
Basic Theoretical Principles.
Requirements.
The Practice of Patch Clamping.
Whole-cell Protocols and Data Analysis.
Single-channel Protocols and Data Analysis.
Further Reading.
Index.
