Biochemical Engineering

Preface
Nomenclature

PART I: Basic Concepts and Principles

INTRODUCTION
Background and Scope
Dimensions and Units
Intensive and Extensive Properties
Equilibria and Rates
Batch vs. Continuous Operation
Material Balance
Energy Balance

ELEMENTA OF PHYSICAL TRANSFER PROCESSES
Introduction
Heat Conduction and Molecular Diffusion
Fluid Flow and Momentum Transfer
Laminar vs. Turbulent Flow
Transfer Phenomena in Turbulent Flow
Film Coefficients of Heat and Mass Transfer

CHEMICAL AND BIOCHEMICAL KINETICS
Introduction
Fundamental Reaction Kinetics

CELL KINETICS
Introduction
Cell Growth
Growth Phases in Batch Culture
Factors Affecting Rates of Cell Growth
Cell Growth in Batch Fermentors and Continuous Stirred Tank Fermentor (CSTF)

PART II: Unit Operations and Apparatus for Bio-Systems

HEAT TRANSFER
Introduction
Overall Coefficients U and Film Coefficients h
Mean Temperature Difference
Estimation of Film Coefficients h
Estimation of Overall Coefficients U

MASS TRANSFER
Introduction
Overall Coefficients K and Film Coefficients k of Mass Transfer
Types of Mass Transfer Equipment
Models for Mass Transfer at the Interface
Liquid Phase Mass Transfer with Chemical Reactions
Correlations for Film Coefficients of Mass Transfer
Performance of Packed Column

BIOREACTORS
Introduction
Some Fundamental Concepts
Bubbling Gas-Liquid Reactors
Mechanically Stirred Tanks
Gas Dispersion in Stirred Tanks
Bubble Columns
Airlift Reactors
Packed-Bed Reactors
Microreactors

MEMBRANE PROCESSES
Introduction
Dialysis
Ultrafiltration
Microfiltration
Reverse Osmosis
Membrane Modules

CELL-LIQUID SEPARATION AND CELL DISRUPTION
Introduction
Conventional Filtration
Microfiltration
Centrifugation
Cell Disruption

STERILIZATION
Introduction
Kinetics of Thermal Death of Cells
Batch Heat Sterilization of Culture Media
Continuous Heat Sterilization of Culture Media
Sterilizing Filtration

ADSORPTION AND CHROMATOGRAPHY
Introduction
Equilibria in Adsorption
Rates of Adsorption into Adsorbent Particles
Single- and Multi-Stage Processes for Adsorption
Adsorption in Fixed Beds
Separation by Chromatography
Biorecognition Assay

PART III: Practical Aspects in Bioengineering

FERMENTOR ENGINEERING
Introduction
Stirrer Power Requirements for Non-Newtonian Liquids
Heat Transfer in Fermentors
Gas-Liquid Mass Transfer in Fermentors
Criteria for Scaling-Up Fermentors
Modes of Fermentor Operation
Fermentors for Animal Cell Culture

INSTRUMENTATION AND CONTROL OF BIOPROCESSES
Introduction
Instrumentation of Bioprocesses
Control of Bioprocesses
Advanced Control of Bioprocesses

DOWNSTREAM OPERATIONS IN BIOPROCESSES
Introduction
Separation of Microorganisms by Filtration and Microfiltration
Separation by Chromatography
Separation in Fixed Beds
Sanitation in Downstream Processes

MEDICAL DEVICES
Introduction
Blood and Its Circulation
Oxygenation of Blood
Artificial Kidney
Bioartificial Liver

Appendix
Index

Prof. Shigeo Katoh is now Professor emeritus at Kobe University, Japan. He was - after his retirement - a full professor at Hanyang University at Korea and a visiting professor at Cheng Kung University at Taiwan. Before his retirement, he was Head of the Bioengineering Laboratory at Kobe University, Japan. He holds a PhD in Chemical Engineering from the University of Kyoto, Japan. Prof. Katoh is an enthusiastic lecturer in Biochemical Engineering, Bioseparation Engineering and Immunotechnology. He is an outstanding expert in biochemical engineering and has published numerous papers in this field.

Prof. Jun-ichi Horiuchi is Director of the Instrumental Analysis Center at the Kitami Institute of Technology (Japan) since 2010. He obtained his Master degree in Engineering from Hokkaido University and thereafter went to the Science University of Tokyo, where he received his PhD in biotechnology in 1995. Afterwards he spent several years as research engineer at Toyo Engineering Corporation, before he became Professor at the Kitami Institute of Technology in 1998. Prof. Horiuchis research interests are in the field of bioprocess control and optimization and up to now he published over 60 peer reviewed jounal contributions, 12 patents and 9 book contributions.

Prof. em. Fumitake Yoshida was one of the world's most respected chemical engineers. Most recently he was professor emeritus of chemical engineering at Kyoto University and well beyond his retirement he continued to be a visiting lecturer at universities around the world. He conducted research on various mass transfer operations, gas-liquid systems in particular, investigating their applications in bioengineering and medical technology. Yoshida received his BS and PhD degrees in engineering from Kyoto University. Among his many awards and citations was election to the National Academy of Engineering of the United States in 1979 for his leadership in chemical, biochemical, and biomedical engineering in Japan.