Fundamental Laboratory Approaches for Biochemistry and Biotechnology

2. Auflage Juni 2009
480 Seiten, Softcover
Wiley & Sons Ltd
Kurzbeschreibung
Written by active scientists, this book provides readers with a logical framework for conducting modern biochemical and molecular biology research. The authors write with passion, and their love of biochemical research shines through on every page.
Ninfa/Ballou/Parsons is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research.
Ninfa/Ballou/Parsons focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves.
Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Chapter 2: Basic Procedures in the Biochemistry Laboratory
Chapter 3: Spectroscopic Methods
Chapter 4: Quantification of Protein Concentration
Chapter 5: Chromatography
Chapter 6: Gel Electrophoresis of Proteins
Chapter 7: Overview of Protein Purification
Chapter 8: Subcellular Fractionation
Chapter 9: Isolation and Characterization of an Enzyme-Alkaline Phosphatase from Escherichia Coli
Chapter 10: Enzyme Kinetics
Chapter 11: Ligand Binding
Chapter 12: Enzymatic Methods of Analysis
Chapter 13: Recombinant DNA Techniques
Chapter 14: Polymerase Chain Reaction (PCR) Technology
Chapter 15: Using the Computer and the Internet for Biochemical Research and Communication