Mass Spectrometry
Principles and Applications
3. Auflage September 2007
502 Seiten, Softcover
Lehrbuch
Kurzbeschreibung
The latest edition of a highly successful textbook, Mass Spectrometry, Third Edition provides students with a complete overview of the principles, theories and key applications of modern mass spectrometry. All instrumental aspects of mass spectrometry are clearly and concisely described: sources, analyzers and detectors. Tandem mass spectrometry is introduced early on and then developed in more detail in a later chapter. Emphasis is placed throughout the text on optimal utilization conditions. Various fragmentation patterns are described together with analytical information that derives from the mass spectra.
The latest edition of a highly successful textbook, Mass Spectrometry, Third Edition provides students with a complete overview of the principles, theories and key applications of modern mass spectrometry. All instrumental aspects of mass spectrometry are clearly and concisely described: sources, analysers and detectors. Tandem mass spectrometry is introduced early on and then developed in more detail in a later chapter. Emphasis is placed throughout the text on optimal utilisation conditions. Various fragmentation patterns are described together with analytical information that derives from the mass spectra.
This new edition has been thoroughly revised and updated and has been redesigned to give the book a more contemporary look. As with previous editions it contains numerous examples, references and a series of exercises of increasing difficulty to encourage student understanding. Updates include: Increased coverage of MALDI and ESI, more detailed description of time of flight spectrometers, new material on isotope ratio mass spectrometry, and an expanded range of applications.
Mass Spectrometry, Third Edition is an invaluable resource for all undergraduate and postgraduate students using this technique in departments of chemistry, biochemistry, medicine, pharmacology, agriculture, material science and food science. It is also of interest for researchers looking for an overview of the latest techniques and developments.
Preface
Introduction
Principles
Diagram of a Mass Spectrometer
History
Ion Free Path
1 Ion Sources
1.1 Electron Ionization
1.2 Chemical Ionization
1.3 Field Ionization
1.4 Fast Atom Bombardment and Liquid Secondary Ion Mass Spectrometry
1.5 Field Desorption
1.6 Plasma Desorption
1.7 Laser Desorption
1.8 Matrix-Assisted Laser Desorption Ionization
1.9 Thermospray
1.10 Atmospheric Pressure Ionization
1.11 Electrospray
1.12 Atmospheric Pressure Chemical Ionization
1.13 Atmospheric Pressure Photoionization (APPI)
1.14 Atmospheric Pressure Secondary Ion Mass Spectrometry (APSIMS)
1.15 Inorganic Ionization Sources
1.16 Gas-Phase Ion-Molecule Reactions
1.17 Formation and Fragmentation of Ions: Basic Rules
2 Mass Analyzers
2.1 Quadrupole Analyzers
2.2 Ion Trap Analyzers
2.3 The Electrostatic Trap or "Orbitrap"
2.4 Time-of-Flight Analyzers
2.5 Magnetic and Electromagnetic Analyzers
2.6 Ion Cyclotron Resonance and Fourier Transform Mass Spectrometry
2.7 Hybrid Instruments
3 Detectors and Computers
3.1 Detectors
3.2 Computers
4 Tandem Mass Spectrometry (MS/MS)
4.1 Tandem Mass Spectrometry in Space or in Time
4.2 Tandem Mass Spectrometry Scan Modes
4.3 Collision-activated or Collision-induced Dissociation (CAD or CID)
4.4 Other Methods of Ion Activation
4.5 Reactions Studied in MS/MS
4.6 Tandem Mass Spectrometry Applications
5 Mass Spectrometry/Chromatography Coupling
5.1 Elution Chromatography Coupling Techniques
5.2 Chromatography Data Acquisition Modes
5.3 Data Recording and Treatment
6 Analytical Information
6.1 Mass Spectrometry Spectral Collections
6.2 High Resolution
6.3 Isotopic Abundances
6.4 Low-mass Fragments and Lost Neutrals
6.5 Number of Rings or Unsaturations
6.6 Mass and Electron Parities, Closed-shell Ions and Open-shell Ions
6.7 Quantitative Data
7 Fragmentation Reactions
7.1 Electron Ionization and Fragmentation Rates
7.2 Quasi-equilibrium and RRKM Theory
7.3 Ionization and Appearance Energies
7.4 Fragmentation Reactions of Positive Ions
7.5 Fragmentation Reactions of Negative Ions
7.6 Charge Remote Fragmentation (CRF)
7.7 Spectrum Interpretation
8 Analysis of Biomolecules
8.1 Biomolecules and Mass Spectrometry
8.2 Proteins and Peptides
8.3. Oligonucleotides
8.4. Oligosaccharides
8.5. Lipids
8.6 Metabolomics
9 Exercises
A. Questions
B. Answers
Appendices
Appendix 1. Nomenclature
1.1. Units
1.2. Definitions
1.3. Analyzers
1.4. Detection
1.5. Ionization
1.6 Ion Types
1.7. Ion-molecule Reaction
1.8. Fragmentation
Appendix 2. Abbreviations
Appendix 3. Fundamental Physical Constants
Appendix 4A . Table of Isotopes in Ascending Mass Order
Appendix 4B. Table of Isotopes in Alphabetical Order
Appendix 5. Isotopic Abundances in % for Various Elemental Compositions CHON (M = 100%)
Appendix 6. Gas-Phase Ion Thermochemical Data of Molecules
Appendix 7. Gas-Phase Ion Thermochemical Data of Radicals
Appendix 8. Literature on Mass Spectrometry
Appendix 9. Mass Spectrometry on Internet
Index
"Overview of the principles, theories, and key applications of modern mass spectrometry." (Materials and Corrosion, November 2007)
