The HPLC Expert
Possibilities and Limitations of Modern High Performance Liquid Chromatography

1. Auflage Mai 2016
357 Seiten, Hardcover
124 Abbildungen (72 Farbabbildungen)
34 Tabellen
Praktikerbuch
Kurzbeschreibung
Hält mit der schnellen Entwicklung der HPLC-Technik Schritt und deckt eine Vielzahl von Themen ab, von der Gradientenoptimierung über Kopplung und 2D-Techniken bis hin zu Dokumentation und Softwaresupport. Vollgepackt mit Insiderwissen von Experten in Industrie und Lehre.
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The rapid development of HPLC instrumentation and technology opens numerous possibilities - and entails new questions. Which column should I choose to obtain best results, which gradient fits to my analytical problem, what are recent and promising trends in detection techniques, what is state of the art regarding LC-MS coupling? All these questions are answered by experts in ten self-contained chapters. Besides these more hardware-related and technical chapters, further related areas of interest are covered: Comparison of recent chromatographic data systems and integration strategies, smart documentation, efficient information search in internet, and tips for a successful FDA inspection. This practical approach offers in a condensed manner recent trends and hints, and will also display the advanced reader mistakes and errors he was not aware of so far.
Pitfalls of LC/MS coupling
Ion chromatography as an application example for LC/MS
Separation of biomolecules by chromatographic techniques
Gradient optimization for RP-HPLC
Gradient prediction
Optimization strategies for RP-HPLC
Integration methods and strategies
HPLC software: performance, comparison, developments
Intelligent documentation of HPLC data for regulated studies
How to survive an FDA inspection
Mining the web for HPLC data
Selecting and comparing HPLC columns
HPLC hardware: recent trends