Applied Molecular Genetics
1. Edition April 1999
XVIII, 294 Pages, Softcover
Wiley & Sons Ltd
Short Description
In this state-of-the-art guide to applied molecular genetics, well-known teacher and author Roger L. Miesfeld presents a clear, no-nonsense approach to the principles underlying molecular genetics and its applications in biological and biomedical research. He addresses the tremendous growth the field has seen in recent years, sorts through the morass of protocols and reports, and gives readers the know-how to combine various molecular techniques to ensure meaningful experimental results. Five major areas are covered, including manipulation of genomic DNA, isolation and characterization of gene coding sequences, the polymerase chain reaction, gene expression in cultured cell systems, and construction of transgenic multicellular organisms.
This text explains the key biochemical and cell biological principles behind some of today's most commonly used applications of molecular genetics, using clear terms and well-illustrated flow schemes. The book is divided into several sections and moves from basic to advanced topics while providing a concise overview of fundamental concepts in modern biotechnology. Each chapter concludes with a Laboratory Practicum describing a hypothetical research objective and the sequence of steps that are most often used to investigate biological questions using molecular genetic methods. In addition, the book provides informative summaries of the latest advances in molecular genetics, using attractive illustrations and a comprehensive reference list.
This text also introduces the use of Internet resources through the World Wide Web as a powerful new tool in molecular genetic research. Seven appendices are included in the book, providing a convenient information resource for properties of nucleic acids, protein and restriction enzymes, a description of common E. coli genetic markers and gel electrophoresis parameters, as well as a list of useful Internet address sites.
Biochemical Basis of Applied Molecular Genetics.
Laboratory Tools for Molecular Genetic Applications.
Overview of Gene Analysis Methods.
CORE METHODS.
Characterization of Genomic DNA.
Isolation and Characterization of Gene Transcripts.
The Polymerase Chain Reaction.
SPECIALIZED APPLICATIONS.
Expression of Cloned Genes in Cultured Cells.
Construction of Transgenic Multicellular Organisms.
Contemporary Applied Molecular Genetics.
Appendices.
