Confocal and Two-Photon Microscopy
Foundations, Applications and Advances
1. Edition November 2001
592 Pages, Hardcover
Wiley & Sons Ltd
Confocal and Two-Photon Microscopy
Foundations, Applications, and Advances
Edited by Alberto Diaspro
Confocal and two-photon fluorescence microscopy has provided
researchers with unique possibilities of three-dimensional imaging
of biological cells and tissues and of other structures such as
semiconductor integrated circuits. Confocal and Two-Photon
Microscopy: Foundations, Applications, and Advances provides clear,
comprehensive coverage of basic foundations, modern applications,
and groundbreaking new research developments made in this important
area of microscopy.
Opening with a foreword by G. J. Brakenhoff, this reference gathers
the work of an international group of renowned experts in chapters
that are logically divided into balanced sections covering theory,
techniques, applications, and advances, featuring:
* In-depth discussion of applications for biology, medicine,
physics, engineering, and chemistry, including industrial
applications
* Guidance on new and emerging imaging technology, developmental
trends, and fluorescent molecules
* Uniform organization and review-style presentation of chapters,
with an introduction, historical overview, methodology, practical
tips, applications, future directions, chapter summary, and
bibliographical references
* Companion FTP site with full-color photographs
* The significant experience of pioneers, leaders, and emerging
scientists in the field of confocal and two-photon excitation
microscopy
Confocal and Two-Photon Microscopy: Foundations, Applications, and
Advances is invaluable to researchers in the biological sciences,
tissue and cellular engineering, biophysics, bioengineering,
physics of matter, and medicine, who use these techniques or are
involved in developing new commercial instruments.
Preface.
Acknowledgements.
Contributors.
The Generalized Microscope (C. Sheppard).
Confocal Microscopy: Basic Principles and Architectures (T.
Wilson).
Two-Photon Microscopy: Basic Principles and Architectures (A.
Diaspro & C. Sheppard).
Cross-Sections of Fluorescent Molecules in Multiphoton Microscopy
(C. Xu).
Resolution and Contrast in Confocal and Two-Photon Microscopy (J.
Jonkman and E. Stelzer).
The Role of Pinhole Size in High-Aperture Two-and Three-Photon
Microscopy (P. Torok & C. Sheppard).
Aberrations and Penetration in In-Depth Confocal and
Two-Photon-Excitation Microscopy (C. de Grauw, et al.).
Group Velocity Dispersion and Fiber Delivery in Multiphoton Laser
Scanning Microscopy (R. Wolleschensky, et al.).
Cellular and Subcellular Perturbations During Multiphoton
Microscopy (K. Konig & U. Tirlapur).
Pracitcal Multiphoton Microscopy (J. Girkin & D. Wokosin).
Sampling, Resolution and Digital Image Processing in the Spatial
and Fourier Domains (K. Castleman).
Image-Restoration Methods: Basics and Algorithms (P. Boccacci
& M. Bertero).
Two-Photon Excitation Fluorescence Microscopy Imaging in Xenopus
and Transgenic Mouse Embryos (A. Periasamy, et al.).
Multiple Color Fluorescence Imaging in Microscopy (K. Castleman).
Stereological Methods for Estimating Geometrical Parameters of
Microscopic Structure by Three-Dimensional Imaging (L. Kubinova, et
al.).
Imaging Live Cells in 3D Using Wide-Field Microscopy with Image
Restoration (W. Carrington).
Confocal Microscopy in the Study of the Cell Nucleus (L. Neri, et
al.).
Confocal Imaging of Neuronal Growth and Morphology in Brain Slices
(I. Skaliora & S. Pagakis).
FISH Imaging (M. Kozubek).
Two-Photon Imaging of Tissue Physiology based on Endogenous
Fluorophores (L. Hsu, et al.).
Two-Photon Near-Infrared Femtosecond Laser Scanning Microscopy in
Plant Biology (U. Tirlapur & K. Konig).
Two-Photon Excitation Microscopy for Image Spectroscopy and
Biochemistry of Tissues, Cells, Organelles, and Lipid Vesicles
Under Physiological Conditions (T. Parasassi, et al.).
Real-Time In Situ Calcium Imaging with Single- and Two-Photon
Confocal Microscopy (K. Fujita & T. Takamatsu).
Superresolution in Fluorescence Confocal Microscopy and in DVD
Optical Storage (R. Pike).
Photobleaching by Confocal Microscopy (J. McNally & C. Smith).
Two-Photon Laser Scanning Microscopy for Characterization of
Integrated Circuits and Optoelectronics (C. Xu).
Index.
that is interested in novel forms of microscopy." (Biomolecular
Engineering, November 2004)
"...well presented with handsome images and figures...a
useful source of reference..." (Annals of Biomedical
Engineering, July 2002)