DNA Transfer to Cultured Cells
Culture of Specialized Cells
1. Edition August 1998
XVI, 296 Pages, Softcover
Wiley & Sons Ltd
Short Description
DNA Transfer to Cultured Cells will describe the different updated methods and applications for transferring genetic material to cells in-vitro. Advances achieved in recent years to develop approaches for introducing DNA into cells have facilitated studies on the mechanism of expression of different important genes in the cells. Consequently, it has been possible to evaluate the role of different genes and their activation in a variety of important processes, such as growth and uncontrolled proliferation (cancer). Different cell types require different schemes for DNA transfer; the book will describe these diverse methods, their application to specific cell types and their relevance to a biological problem.
DNA transfer to cultured cells
Edited by Katya Ravid and R. Ian Freshney
Rapid advances in DNA transfer technology have transformed many disciplines, ranging from molecular genetics to biotechnology. Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. It is now possible to regulate cell growth that may lead to cancer, develop new biopharmaceuticals, and apply knowledge about the role of genes in cell processes to basic research in molecular genetics.
DNA Transfer to Cultured Cells is the first quick reference to all of the established techniques for the transfer of genetic material to cells in vitro. Featuring contributions by leading researchers in the field, this detailed guide walks the reader through a variety of DNA transfer methods, describes their application to specific cell types, and integrates aspects of molecular biology with tissue culture. Offering overviews and detailed protocols for the techniques under discussion in each of its sections, this book covers an exceptionally broad array of topics, including:
* Viral infection
* Electroporation
* Phosphate precipitation
* DEAE Dextran
* Liposomes
* Yeast artificial chromosomes (YACs)
* Whole chromosome transfer
* Enhanced expression.
Special sections at the end of each chapter list suppliers for necessary reagents and materials. This easy-to-use, self-contained guide addresses key developments of recent years as well as emerging trends in DNA transfer. For practical applications in cell biology, genetics, heredity, biotechnology, or evolution, DNA Transfer to Cultured Cells is a unique and unparalleled resource.
Production and Use of Retroviruses (G. Hicks, et al.).
Electroporation of DNA into Cultured Cell Lines (L. Cataldo, et al.).
Transfection of Rat Adipose Cells by Electroporation (M. Quon).
Calcium Phosphate Transfection (K. Conn. et al.).
Strontium Phosphate Transfection (R. Reddel, et al.).
DEAE- Dextran Transfection (J. Schwartz & R. Rosenberg).
Cationic Liposomes (V. Bichko).
Transfection of Mammalian Cells with Yeast Artificial Chromosomes. (W. Strauss).
Mapping Human Senescence Genes Using Microcell-Mediated Chromosome Transfer (R. Newbold & A. Cuthbert).
Methotrexate and DHFR Amplification (M. Ricketts).
List of Suppliers.
Index.
