Molecular Biology in Cellular Pathology
1. Edition April 2003
400 Pages, Hardcover
Handbook/Reference Book
Short Description
The latest edition of this highly successful text covers the major advances in the methods used in cellular and molecular pathology. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology.
The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology. Color plates are a new feature of this edition, illustrating the advances in fluorescence labeling of cells.
Preface to Molecular Biology in Histopathology
Contributors
BLOTTING TECHNIQUES: METHODOLOGY AND APPLICATIONS
Introduction
Blotting techniques
References
IN SITU HYBRIDISATION IN HISTOPATHOLOGY
Introduction
Experimental conditions
Probes and labels
Controls and pitfalls
Double-labelling
Increasing the sensitivity of ISH
What we do in our laboratories
Applications of ISH: examples
Perspective
References
DNA FLOW CYTOMETRY
Introduction
Definitions and terms
Dye used for DNA analysis
Sample preparation for DNA analysis
Analysis of the DNA histogram
Quality control
Computer analysis of the DNA histogram
Multiparametric measurement
Acknowledgements
References
INTERPHASE CYTOGENICS
Introduction
Interphase cytogenics
Applications
Conclusion
References
ONCOGENES
Introduction
Identification of the oncogenes
Functions of the oncogenes
Mechanism of oncogene activation
Oncogenes in colorectal cancer
Oncogenes in breast cancer
Oncogenes in lung cancer
Oncogenes in haematological malignancies
Other cancers
Conclusion
References
MOLECULAR AND IMMUNOLOGICAL ASPECTS OF CELL PROLIFERATION
The cell cycle and its importance in clinical pathology
Molecular control of the cell cycle
Cell cycle control
The cell cycle and cancer
Immunocytochemical markers of proliferating cells
References
Further reading
INTERPHASE NUCLEOLAR ORGANISER REGIONS IN TUMOUR PATHOLOGY
Introduction
The AgNORs
NOR silver-staining
Quantitative AgNOR analysis
AgNORs as a parameter of the level of cell proliferation
Application of the AgNOR technique to tumour pathology
What future for AGNORs in tumour pathology
References
APOPTOSIS AND CELL SENESCENCE
Introduction
Apoptosis
Cell senescence
Summary
References
THE POLYMERASE CHAIN REACTION
Introduction
Principles
Analysis of products
RT-PCR
Quantitative PCR
DNA and RNA extraction
Correlation of the PCR with morphology
Problems
Applications
Diagnostic applications
Infectious diseases
Identity
The future
References
Online information
LASER CAPTURE MICRODISSECTION: TECHNIQUES AND APPLICATIONS IN THE MOLECULAR ANALYSIS OF THE CANCER CELL
Introduction
The principle of LCM
Technical considerations
Advantages and disadvantages of LCM
Applications of LCM
Future perspectives
Acknowledgements
References
THE IN SITU POLYMERASE CHAIN REACTION
Introduction
Overview of the methodology
In-cell PCR technologies
In-cell amplification of DNA
In-cell amplification of RNA
References
TAQMAN(R) TECHNOLOGY AND REAL-TIME POLYMERASE CHAIN REACTION
Introduction
Probe technologies
TaqMan(r) probe and chemistry
TaqMan(r) PCR conditions
Interpretation of results
Relative quantitation
References
GENE EXPRESSION ANALYSIS USING MICROARRAYS
Introduction
Microarray experiments
Data analysis
Recent examples of microarray applications
Conclusions
Acknowledgements
References
Further reading
Useful websites
COMPARATIVE GENOMIC HYBRIDISATION IN PATHOLOGY
Introduction
Technique
Data analysis
Applications
Clinical applications
Screening for chromosomal abnormalities in fetal and neofetal genomes
Future perspectives
References
DNA SEQUENCING AND THE HUMAN GENOME PROJECT
Introduction
DNA sequencing: the basics
Applications of DNA sequencing
The Human Genome Project
Further reading
Useful websites
MONOCLONAL ANTIBODIES: THE GENERATION AND APPLICATION OF 'TOOLS OF THE TRADE' WITHIN BIOMEDICAL SCIENCE
Introduction
Antibodies and antigens
Polyclonal antibodies
Monoclonal antibody development
Monoclonal antibody variants
Monoclonal antibody applications
Specific applications
Therapy
Specific applications
Conclusions
Acknowledgements
References
PROTEOMICS
Introduction
Definitions and applications
Stages in proteome analysis
Future directions
References
Index