Cell-free Protein Synthesis
Methods and Protocols
1. Edition October 2007
XX, 242 Pages, Hardcover
93 Pictures (12 Colored Figures)
14 tables
Practical Approach Book
Short Description
The most up-to-date guide to the field covers latest advances including membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale.
Buy now
Price: 135,00 €
Price incl. VAT, excl. Shipping
Euro prices for Wiley-VCH and Ernst & Sohn titles are only valid for Germany. In EU countries, local VAT applies. Postage will be charged.
- Out of print -
Further versions
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
The constructive approach for cell-free translation (T. Ueda)
Functional genomic analysis using sequential cell-free protein synthesis (J. R. Swartz)
Cell-free technology for rapid production of patient-specific fusion protein vaccines (A. R. Goerke, J. Yang, G. Kanter, R. Levy and J. R. Swartz)
Bacterial cell-free system for highly efficient protein synthesis (T. Kigawa, T. Matsuda, T. Yabuki, S. Yokoyama)
The use of the Escherichia-coli cell-free protein synthesis for structural biology and structural proteomics (T. Kigawa, M. Inoue, M. Aoki, T. Matsuda, T. Yabuki, E. Seki, T. Harada, S. Watanabe, S. Yokoyama)
The wheat germ cell-free protein synthesis system (T. Sawasaki, Y. Endo)
Cell-free expression of integral membrane proteins for structural studies (C. Klammt, D. Schwarz, I. Lehner, S. Sobhanifar, F. Löhr, J. Zeelen, C. Glaubitz, V. Dötsch, F. Bernhard)
Cell-free production of membrane protein in presence of detergents (J. Betton, M. Miot)
Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering (H. Nakano, T. Yamane)
Gene Cloning and Expression in Molecular Colonies (A. B. Chetverin, T. R. Samatov, H. V. Chetverina)
James Swartz is Professor of Chemical Engineering at Stanford University, California, USA. A graduate of the Massachusetts Institute of Technology, he has pioneered protein synthesis on an industrial scale while working for the companies Eli Lilly and Genentech. He is a leading expert on the biotechnological synthesis of mammalian proteins using bacterial whole cells and cell extracts.
