X-Ray Crystallography of Biomacromolecules
A Practical Guide
1. Edition November 2006
XIII, 305 Pages, Hardcover
150 Pictures (10 Colored Figures)
2 tables
Practical Approach Book
Short Description
Written by one of the main contributors to progress in protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. It covers the theory, practice and latest achievements in x-ray crystallography, such that any researcher in structural biology will benefit from the contents.
- Out of print -
Further versions
Written by one of the most significant contributors to the progress of protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. It covers the theory, practice and latest achievements in x-ray crystallography, such that any researcher in structural biology will benefit from this extremely clearly written book.
Part A covers the theoretical basis and such experimental techniques as principles of x-ray diffraction, solutions for the phase problem and time-resolved x-ray crystallography. Part B includes case studies for different kinds of x-ray crystal structure determination, such as the MIRAS and MAD techniques, molecular replacement, and the difference Fourier technique.
Introduction
Techniques
Principles of X-ray Diffraction by a Crystal
Methods for Solving the Phase Problem
Model Building and Refinement
Time-resolved X-ray Crystallography
PART B CASE STUDIES FOR DIFFERENT KINDS OF X-RAY CRYSTAL STRUCTURE DETERMINATION OF BIOMACROMOLECULES
Space Group Determination and Data Reduction
Phase Determination by MIRAS Technique
Phase Determination by MAD Technique
Phase Determination by Molecular Replacement
Difference Fourier Technique
Map Improvement by NCS Averaging and other Methods
Automated Model Building
Structure Validation and PDB-Submission
His main reserach interest is in the determination of X-ray structures of metalloproteins and -enzymes, PLP-dependent enzymes, CoA-transferases and other proteins. His group is part of the department of X-ray structure determination of proteins headed by nobel laureate Robert Huber.
