Introduction to Biological Imaging
1. Edition May 2024
352 Pages, Softcover
Textbook
Introduction to Biological Imaging
Discover what biological imaging is able to accomplish in this up-to-date textbook
One of the fundamental goals of biology is to understand how living organisms establish and maintain their spatiotemporal organization of the biochemical, cell biological and developmental biology processes that sustain life. Biological systems are inherently complex with a large number of components needed to sustain cellular function. In order to understand any complex system, one must determine its composition by identifying the components it is made of, how each of these components function and carry out their specific task, and how they interact with one another to function together. To grasp the link of such changes to physiological cell and tissue function and/or pathogenesis/disease progression, we need to understand how modifications alter macromolecular function, macromolecular interactions, and/or spatiotemporal distribution and overall supramolecular structural organization. Biological imaging holds the key to understanding spatiotemporal organization, and will thus be increasingly important for the next generations of biological and biochemical researchers.
Introduction to Biological Imaging provides the first comprehensive textbook surveying this subject. It elucidates the fundamental principles underlying the capture and production of bioimages, the requirements of image analysis and interpretation, and some key problems and solutions in bioimaging. It includes everything experimental biologists need to incorporate appropriate bioimaging solutions into their work.
Introduction to Biological Imaging readers will also find:
* Coverage of all major types of biological imaging, including medical imaging, cellular imaging, macromolecular imaging, and more
* Advice on preparing samples for various imaging methods
* Specific examples in each chapter connecting bioimaging process to the production of real experimental data
Introduction to Biological Imaging is a valuable introduction for undergraduate or graduate students in courses relating to bioimaging, as well as scientists and researchers in the biological and medical fields who want a one-stop reference for the full range of imaging techniques.
2. Basics of Atomic and Molecular Physics 2.1. Matter and energy 2.2. Electromagnetic spectrum 2.3. Interaction of radiation with matter
3. Basics of Optics 3.1. Properties of light 3.2. Reflection 3.3. Refraction 3.4. Dispersion 3.5. Lenses 3.6. Numerical aperture 3.7. Scattering 3.8. Interference 3.9. Diffraction 3.10. Airy disk 3.11. Point spread function 3.12. Resolution 3.13. Aberrations 3.14. Polarization 3.15. Photoelectric effect 3.16. Valence electron transitions 3.17. Vibrational and rotational transitions 3.18. Stimulated emission
4. Basics of Fourier "Math"
5. Basics of Imaging and Image Formation 5.1. Goals of imaging 5.2. Source of radiation 5.3. Beam collimation 5.4. Optical lenses 5.5. Lens-free imaging/near-field 5.6. Signal detection and detectors 5.7. Modulation-, contrast- and optical-transfer functions 5.8. Field of view, coverage 5.9. Flood-beam versus spot-scan imaging 5.10. Radiation damage 5.11. Spectroscopic Imaging 5.12. Multi-dimensional and multi-channel imaging 5.13. Sample preparation requirements 5.14. Labels and labeling 5.15. Label-free imaging 5.16. 2D versus 3D imaging 5.17. Structural versus functional imaging 5.18. Correlative imaging
6. Basics of Image Processing and Image Analysis 6.1. Digital Images 6.2. Image restoration, enhancement and reconstruction 6.3. Visualization 6.4. Image segmentation 6.5. Measurements/quantitative analysis
7. Techniques for Macromolecular Structure Determination 7.1. X-ray Crystallography 7.2. Cryo-Electron Microscopy 7.3. NMR spectroscopy 7.4. Small angle scattering: SAXS & SANS 7.5. Atomic Force Microscopy (AFM)
8. Techniques for Cellular Ultrastructure: Architectural Imaging 8.1. Overview 8.2. Historical Perspective/Milestones 8.3. Theoretical Background 8.4. Sample Preparation 8.5. Instrumentation 8.6. Data Collection and 3D Reconstruction 8.7. Data Visualization and Analysis
9. Techniques for Cells and Tissues: Characterization and Localization Imaging 9.1. Overview 9.2. Historical Perspective/Milestones 9.3. Theoretical Background 9.4. Instrumentation/Experimental Setup 9.5. Data Collection 9.6. Data Analysis 9.7. Visualization
10. Techniques for Cell and Tissue Chemical Imaging 10.1. X-ray Microanalysis 10.2. Raman Imaging 10.3. FTIR 10.4. Mass Spectrometry
11. Techniques for Tissue and Organ Medical Imaging 11.1. Positron Emission Tomography (PET) 11.2. Computed (Axial) Tomography 11.3. Magnetic Resonance Imaging (MRI) 11.4. Medical Ultrasound 11.5. Bioluminescence 11.6. Optical Coherence Tomography (OCT) 11.7. Histology/Histopathology
12. Future of Bioimaging 12.1. Future of Macromolecular Imaging 12.2. Future of (Sub)Cellular and Tissue Imaging 12.3. Future of Chemical Imaging 12.4. Future of Medical Imaging 12.5. Future of Image Processing and Image Analysis 12.6. Future of Multiscale Imaging 12.7. Future of Multimodal Imaging - Correlative Imaging 12.8. Future of Information Integration - Multiscale, Multimodal and Integrated
